Aston university

Research project consisted of a 10-week project to study transmembrane protein, tetraspanin CD81 as a potential drug target for the Hepatitis C virus. CD81 was recombinantly produced in the methylotrophic yeast Pichia pastoris. The expression was identified by western blot analysis using monoclonal antibodies against different epitopes of the protein. Expressed CD81 was solubilised using an array of detergents to determine the most efficient one. CD81 produced, was shown to interact with… Show more Research project consisted of a 10-week project to study transmembrane protein, tetraspanin CD81 as a potential drug target for the Hepatitis C virus. CD81 was recombinantly produced in the methylotrophic yeast Pichia pastoris. The expression was identified by western blot analysis using monoclonal antibodies against different epitopes of the protein. Expressed CD81 was solubilised using an array of detergents to determine the most efficient one. CD81 produced, was shown to interact with Hepatitis C virus E2 glycoprotein, binding was confirmed using an ELISA. Show less

Aston university

Volunteer

Associated with Aisha Azhar

Peer Advisor

Associated with Aisha Azhar

Professional Mentoring Scheme

Associated with Aisha Azhar

Exchange Student

Associated with Aisha Azhar