Nazreen Abdul Muthaliff

Experience

• 

  A*STAR - Agency for Science, Technology and Research

  Sept 2008 - Oct 2008

  Intern

  Supervisor Dr Choong Meng Ling• Aided supervisors in experiments and independently performed routine experiments • Techniques: Cell culture, qRT-PCR, PAGE, Western blot,

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  Singapore Polytechnic

  Apr 2009 - Jan 2010

  Final Year Student

  Final Year Project under Mr Wang Baoshuang, SP • Studied the methylation pattern in CpG sites in Cyr61 • Explored the possible involvement of p53 in the methylation process.• Obtained a Distinction for the project, which was presented during the Young Scientists’ Symposium, 2010• Techniques: Cell culture, qRT-PCR, bisulphite sequencing

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  A*STAR - Agency for Science, Technology and Research

  May 2011 - Jul 2011

  Intern

  Laboratory of Dr Ng Huck Hui• Coordinated with supervisor Dr Chuti Laowtammathron in performing basic experiments• Techniques: Cell culture, qRT-PCR, DNA gel electrophoresis

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  A*STAR - Agency for Science, Technology and Research

  Dec 2011 - Jan 2012

  Intern

  Epigenetics and Cell Fate Laboratory under Dr Jonathan Loh Yuin-Han• Successfully cloned plasmids for a proposed dual reporter project• Coordinated with mentor Dr Delon Toh and aided some of his experiments• Techniques: Cell culture, cloning

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  A*STAR - Agency for Science, Technology and Research

  May 2012 - Apr 2013

  Project Student (UROPS)

  Undergraduate Research Opportunities Programme in Science (UROPS), Dr Jonathan Loh Yuin-Han, IMCB • Studied gene expression changes in embryonic stem cells upon differentiation• Optimzed immunofluorescence staining for embryoid bodies, allowing it to be used for upcoming projects• Set-up Resazurin virus titering assay, easing quantitation of viruses produced in the lab regularly• Supported colleagues in their experiments• Techniques: Cell culture, cloning, qRT-PCR, Immunofluorescence Show less

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  National University of Singapore

  May 2013 - May 2014

  Final Year Student

  - Under Associate Professor Gan Yunn Hwen, NUS - Deciphering the role of exogenous Hcp1 of Burkholderia pseudomallei on multinucleated giant cell formation as part of mentor’s PhD project. - Techniques: Cell culture, bacterial maintenance, protein purification, PAGE

• 

  A*STAR - Agency for Science, Technology and Research

  Aug 2014 - Jun 2017

  Research Officer

  • Involved in studying gene regulation in Embryonic stem cells and keratinocytes• Established mouse induced pluripotent stem cells (miPSC) protocol • Supporting colleagues in their experiments• Laboratory maintenance and purchasing • Techniques : Cell culture, cloning, DNA gel electrophoresis, protein gel electrophoresis (PAGE), Western blot, virus production, quantitative real-time PCR (qRT-PCR)

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  A*STAR - Agency for Science, Technology and Research

  Jan 2018 - now

  - Enzyme library creation- Site-saturation mutagenesis - Combinatorial saturation mutagenesis- Utilizing robots to automate- Protein production and purification - Culturing yeast cells for producing high value compounds- Creating DNA library in order to increase yield - Creating knock-outs and knock - ins using CRISPR technology- Utilizing GC-MS and HPLC for analysis- Design of Experiments for optimization

  • Senior Research Officer

    Apr 2022 - now

  • Research Officer

    Jan 2018 - Apr 2022