
Gunjan Sharma
Postdoctoral Research Associate

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About me
Plant Molecular Scientist | Project Manager | Plant Proteolysis Researcher
Education

National Institute of Plant Genome Research, Jawaharlal Nehru University, India
2010 - 2016Doctor of Philosophy - PhD Plant Molecular Biology and Biotechnology1. Extensive experience of plant molecular biology techniques, transgenic generation in Arabidopsis and abiotic stress response assessment at molecular, biochemical and physiological level.2. Protein purification and in vitro E3 ubiquitin ligase activity of rice proteins. Confocal microscopy for studying fluorescent tagged proteins in Arabidopsis roots, protoplasts, tobacco and onion epidermal cells.Plant Techniques: Arabidopsis handling (transformation, screening transformants and… Show more 1. Extensive experience of plant molecular biology techniques, transgenic generation in Arabidopsis and abiotic stress response assessment at molecular, biochemical and physiological level.2. Protein purification and in vitro E3 ubiquitin ligase activity of rice proteins. Confocal microscopy for studying fluorescent tagged proteins in Arabidopsis roots, protoplasts, tobacco and onion epidermal cells.Plant Techniques: Arabidopsis handling (transformation, screening transformants and their abiotic stress response assessment at molecular and physiological levels), rice tissue culture, seed germination assays, assay, Fv/Fv measurements, relative water content measurement in water deficit stress treated plants proline, soluble sugars and MDA estimation, ion leakage estimation Growth of rice under salt, mannitol and ABA and submergence stress in growth chamber and glass house setting. Ferrozine assay Show less

Banasthali University
2008 - 2010Masters Biotechnology
Experience

NATIONAL INSTITUTE OF PLANT GENOME RESEARCH
May 2016 - Sept 2016Postdoctoral Research AssociateFunctional characterization of SAP gene family in rice.

University of Nottingham
Sept 2016 - Mar 2022Research FellowFinding novel oxygen sensors in plants (Arabidopsis and rice). I work to understand the post translational regulation of these sensor proteins. They are Met-Cys-initiating proteins and continuously degraded under normal/unstressed conditions via N-degron pathway. I use extensive biochemical approaches to understand the protein chemistry and physiological/phenotyping approaches to functionally characterise these proteins. Skills acquired:TechnicalWestern blotting, Affinity purification, Co-IP and CHIP, site-directed mutagenesis, inverse PCR deletion construct generation, in vitro translation of Arabidopsis/rice proteins in rabbit reticulocyte lysate and wheat germ extract, cell free degradation assay, in vitro E3 ubiquitin ligase activity assay. CRISPR-Cas9 construct preparation. Primary root tip survival assay under hypoxia, hypocotyl angle, PCH and ALA synthesis rate.Supervision of PhD student and research technicians. Show less

University of Birmingham
Apr 2022 - Jun 2023Postdoctoral Researcher
University of Nottingham
Jun 2023 - Mar 2024Project Manager
University of Birmingham
Apr 2024 - nowPostdoctoral Research Fellow
Licenses & Certifications

Associate Fellow of Higher Education
Advance HE Fellowship CertificateJul 2025
Project Management (online course)
University of OxfordOct 2023- View certificate

Project Management Foundations
LinkedInMar 2023
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