Gunjan Sharma

Gunjan Sharma

Postdoctoral Research Associate

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  • Timeline

  • About me

    Plant Molecular Scientist | Project Manager | Plant Proteolysis Researcher

  • Education

    • National Institute of Plant Genome Research, Jawaharlal Nehru University, India

      2010 - 2016
      Doctor of Philosophy - PhD Plant Molecular Biology and Biotechnology

      1. Extensive experience of plant molecular biology techniques, transgenic generation in Arabidopsis and abiotic stress response assessment at molecular, biochemical and physiological level.2. Protein purification and in vitro E3 ubiquitin ligase activity of rice proteins. Confocal microscopy for studying fluorescent tagged proteins in Arabidopsis roots, protoplasts, tobacco and onion epidermal cells.Plant Techniques: Arabidopsis handling (transformation, screening transformants and… Show more 1. Extensive experience of plant molecular biology techniques, transgenic generation in Arabidopsis and abiotic stress response assessment at molecular, biochemical and physiological level.2. Protein purification and in vitro E3 ubiquitin ligase activity of rice proteins. Confocal microscopy for studying fluorescent tagged proteins in Arabidopsis roots, protoplasts, tobacco and onion epidermal cells.Plant Techniques: Arabidopsis handling (transformation, screening transformants and their abiotic stress response assessment at molecular and physiological levels), rice tissue culture, seed germination assays, assay, Fv/Fv measurements, relative water content measurement in water deficit stress treated plants proline, soluble sugars and MDA estimation, ion leakage estimation Growth of rice under salt, mannitol and ABA and submergence stress in growth chamber and glass house setting. Ferrozine assay Show less

    • Banasthali University

      2008 - 2010
      Masters Biotechnology
  • Experience

    • NATIONAL INSTITUTE OF PLANT GENOME RESEARCH

      May 2016 - Sept 2016
      Postdoctoral Research Associate

      Functional characterization of SAP gene family in rice.

    • University of Nottingham

      Sept 2016 - Mar 2022
      Research Fellow

      Finding novel oxygen sensors in plants (Arabidopsis and rice). I work to understand the post translational regulation of these sensor proteins. They are Met-Cys-initiating proteins and continuously degraded under normal/unstressed conditions via N-degron pathway. I use extensive biochemical approaches to understand the protein chemistry and physiological/phenotyping approaches to functionally characterise these proteins. Skills acquired:TechnicalWestern blotting, Affinity purification, Co-IP and CHIP, site-directed mutagenesis, inverse PCR deletion construct generation, in vitro translation of Arabidopsis/rice proteins in rabbit reticulocyte lysate and wheat germ extract, cell free degradation assay, in vitro E3 ubiquitin ligase activity assay. CRISPR-Cas9 construct preparation. Primary root tip survival assay under hypoxia, hypocotyl angle, PCH and ALA synthesis rate.Supervision of PhD student and research technicians. Show less

    • University of Birmingham

      Apr 2022 - Jun 2023
      Postdoctoral Researcher
    • University of Nottingham

      Jun 2023 - Mar 2024
      Project Manager
    • University of Birmingham

      Apr 2024 - now
      Postdoctoral Research Fellow
  • Licenses & Certifications

    • Associate Fellow of Higher Education

      Advance HE Fellowship Certificate
      Jul 2025
    • Project Management (online course)

      University of Oxford
      Oct 2023
    • Project Management Foundations

      LinkedIn
      Mar 2023
      View certificate certificate