MARGARET PHILLIPS

Experience

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  Isabella Thoburn College - India

  Jan 2006 - Jan 2007

  Guest Lecturer

  Inorganic Chemistry Classes for under graduates and post graduates

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  ISABELLA THOBURN P.G. COLLEGE

  May 2007 - Jul 2008

  Part-Time Lecturer

  Coordinated practical and theory classes in Chemistry, for undergraduate and graduate students.

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  Nanyang Technological University Singapore

  Dec 2009 - Sept 2016

  During my Ph.D training I gained hands on experience in expression and purification of membrane proteins from E.coli media, involving ultracentrifugation, membrane solubilization, Ni-NTA histidine tag purification, FPLC and SDS-PAGE. I have also used techniques such as Circular Dichroism and Thermaflour Assay for protein secondary structure determination. My main focus however was using NMR to establish the global fold of the proteins in solution as well as identify protein-ligand interaction sites. I used solid-state NMR to characterize a small molecule inhibitor for a tetrameric water channel membrane protein. Show less

  • Research Associate

    Sept 2015 - Sept 2016

  • Doctor of Philosophy

    Aug 2011 - May 2016

  • Research Associate

    Dec 2009 - Jul 2011
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  Nanyang Technological University

  Jun 2017 - Jun 2019

  Postdoctoral Research Fellow

  As a Research Fellow I have gained more experience in data acquisition and data analysis in solid-state NMR. I am involved in preparing samples and performing routine NMR experiments to study the structure and functions of different proteins. i have used Transmission Electron Micorscopy to study the mechansim of amyloid fibril disaggregation by chaperones. I am incharge of laboratory purchases and safety management.

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  Albany College of Pharmacy and Health Sciences

  Jul 2019 - Jun 2021

  Postdoctoral Researcher

  I worked on the structural and biophysical characterization of various bromodomains, including ATAD2/B and BRPF1 proteins, using my NMR expertise to identify and characterize the binding sites of mono- and diacetylated histone ligands. New skills acquired included ITC and X-ray crystal screen setup and data analysis for solving high resolution crystal structure of bromodomains in complex with histone ligands.

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  University of Vermont

  Jun 2021 - Jul 2023

  Postdoctoral Research Associate

  My focus is on structural and functional characterization of bromodomain-containing proteins and how they select for specific histone modifications. New Skills acquired during this time include mammalian cell culture, RT-qPCR, immunoblotting. Hands on training in Cryo-EM and received Merit badges in Vitrobot use for cryo grid preparation, clipping of cryo grids and screening using Glacios microscope. Currently being trained in use of Leginon and CryoSpark softwares for data analysis.

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  St. Jude Children's Research Hospital

  Jul 2023 - now

  NMR Scientist