Hannah Biederman (Wickham)

Experience

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  Wickham Veterinary Clinic

  Apr 2012 - May 2015

  Veterinary Assistant

  •Assisted in various medical procedures•Participated in treatment of patients under supervision•Received instruction in veterinary care

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  Henry County Extension Office

  May 2015 - Aug 2015

  STEM Instructor

  Designed projects for kids to encourage interest in STEM fields

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  Iowa State University

  Jan 2016 - May 2016

  Undergraduate Research Assistant in Dr. Sakaguchi's Lab

  • Developed basic research techniques studying neural progenitor cell migration in zebrafish• Constructed multiple figure plates on Adobe Photoshop of neural progenitor cell migration• Created and presented a group poster on research• Refined ability to read and understand scientific literature

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  Monsanto Company

  May 2016 - Jul 2016

  Field Inspector

  • Managed data on corn growth stages to prevent pollination and maintain sterile fields• Judged field fertility quickly and accurately under time restriction

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  Iowa State University

  May 2017 - Jun 2021

  • Refined skills on plasmid isolations, sample preparation, western blot, ICC, and mammalian cell culture.• Communicated scientific findings to coworkers, management, and the public clearly and accurately.• Worked carefully with a variety of toxicants and performed independent experiments on cell specific toxicity.• Assisted with mice treatments, dissections, perfusions, and tests including Morris Water Maze, Versamax, and novel scent test.• Used Microsoft Word and PowerPoint to showcase compiled data analyzed via GraphPad.• Recorded daily detailed notes on experiments. Show less • Carried out general lab techniques such as ligations, transformations, plasmid isolations, PCR purification, DAB staining, quantitative PCR set up, Slot Blot, sample preparation, and Western Blot• Passaged cells and plated in flasks/ 26 well plates/ 96 well plates, created stocks, changed media, treated cells with Rotenone and Puromycin, and restarted culture from stock• Trained in general handling of mice under supervision. Removed nasal cavities, helped with dissections, and assisted with making brain slice cultures. Tested tissue samples via IHC deparaffinization (colon sections)• Participated in general care of zebrafish and sorted GFP embryos. Created lysates and fixed fish on slides under supervision Show less

  • Researcher

    Aug 2019 - Jun 2021

  • Undergraduate Research Assistant in Dr. Kanthasamy's Lab

    May 2018 - Aug 2019

  • Undergraduate Research Assistant in Dr. Trimarchi's Lab

    Jan 2017 - May 2018

  • Undergraduate Field Work in Dr. Volbrect's Lab

    May 2017 - Jul 2017
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  Veterinary Diagnostics Lab (Molecular), Iowa State University

  Jun 2021 - Nov 2021

  Diagnostic Associate

  • Refined skills on PCR• Analyzed PCR results and communicated them to the client

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  Iowa State University

  Dec 2021 - Feb 2023

  Research Scientist II

  • Troubleshot and optimized immunofluorescent protocols for organoids with non-species-specific antibodies.• Created and modified protocols for immunofluorescence and light sheet microscopy for organoids.• Executed R21 grant requirements in a timely manner through independent experiment design and implementation.• Gained experience in organoid culture and isolation from tissue.• Designed and carried out chemotherapeutic toxicity assays and ELISAs with organoids and supernatants.• Acquired skills in RNA In Situ Hybridization.• Trained in general handling of canines for sample collection.• Maintained a thorough and up to date laboratory notebook.• Communicated research results to various collaborators and presented results at ISSCR 2022 conference. Show less

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  Contract worker for Boehringer Ingelheim

  Feb 2023 - May 2024

  Research Scientist I