Varsha Kumari

Experience

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  National Chemical Laboratory

  Nov 2016 - May 2017

  Trainee
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  IISER Bhopal

  Jan 2018 - Jan 2019

  Junior Research Fellow

  • Designed SOPs and its routine operation in in-vitro protein studies.• Create mutant libraries employing plasmid manipulation and PCR, followed by screening positive clones and sequencing.• Sequence analysis and maintenance of samples via glycerol stocks.• Gene overexpression and isolation followed by membrane protein expression and extraction via biophysical experiments• Study of protein behaviour in-vitro employing techniques including protein quantification by nanodrop, SDS-PAGE, UV/Vis spectroscopy and 96-well plates based fluorescence assays Show less

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  University of Hyderabad

  Oct 2019 - Mar 2022

  Research Fellow

  •Experimental design, execution and troubleshooting of lab protocols in addition to scientific writing skills •Construction and cloning of DNA and plasmids via PCR in addition to using sequence analysis tools including UCSC genome browser, CLUSTAL sequence alignment and UniProt•Maintaining a database of DNA stocks of yeast and bacterial cultures•Detailed maintenance of data and inventories of lab materials•Data analysis using standard software packages including BioEdit, SigmaPlot, ImageJ, Origin, BioLabs, Pymol etc•Execution of upstream (cell harvest, lysis, clarification) and downstream (Centrifugation, filtration, concentration) processes.•Protein analytical methods (Eg. protein quantitation by BCA and Bradford, detection / quantitation of process-related impurities such as host-cell protein expression and nucleic acids)•Study of protein behaviour in-vitro employing techniques including UV/Vis spectroscopy, SDS-PAGE, Western blots, Fluorescence microscopy and Chemiluminescence enzyme immunoassays Show less

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  Xencor

  Jan 2023 - Nov 2023

  Research Associate 1

  •Collaborate with team members to design, execute, and analyze experiments by utilizing effective experimental design and data interpretation techniques.•Accurately document experimental protocols, designs, and results in a laboratory notebook with precise detail to ensure reproducibility and compliance.•Conduct and troubleshoot a variety of cloning experiments in support of research, ensuring all procedures are in compliance with established protocols and regulations.•Expertly execute standard operating procedures to ensure consistency and accuracy across experiments.•Maintain laboratory inventory, equipment, and supplies to ensure laboratory operations run smoothly and efficiently.•Utilize computer programs including Sequencher and Geneious Prime to collect, analyze, and record data with precision and accuracy.•Analyze and report data on established timelines, ensuring adherence to project goals and objectives.•Participate in departmental meetings to share data and collaborate on research-related tasks across multiple groups.•Perform other duties as required to support the overall success of the team and department•Trained and mentored new team members on Good Laboratory Practices (GLP), safety guidelines, practices, experimental execution, and documentation. Show less

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  Duke University Health System

  Nov 2023 - now

  Lab Research Analyst