Joselito Lopez

Experience

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  GenPharm International, Inc.

  Jun 1990 - Nov 1997

  Research Associate

  Performed the construction of an expression vector system incorporating synthetic immunoglobulin genes generated through PCR. The resulting construct was used for the production of the recombinant antibodies that became the company’s first entirely human antibody product (HuMax-CD4) to enter Phase I clinical trials for the treatment of rheumatoid arthritis (following the acquisition of GenPharm by Medarex in 1997).

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  Medarex, Inc.

  Nov 1997 - Jan 2010

  Research Associate 3

  Member of a small molecular biology group that was tasked with developing a novel expression system to produce recombinant antibodies. The resulting expression system produced (anti-CTLA4) antibodies that eventually became known as ipilimumab, subsequently becoming an FDA approved immunotherapy for the treatment of metastatic melanoma (following the acquisition of Medarex by Bristol-Myers Squibb in 2010).

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  Bristol-Myers Squibb Company, Biologics Discovery California

  Jan 2010 - Mar 2014

  Research Associate 3

  Tasked with producing high quality pure DNA suitable for pronuclear microinjection to achieve the overall goal of creating new transgenic models for the company’s HuMab platform. Developed PCR genotyping assays and antigen-specific ELISA procedures to characterize new transgenic models. Developed successful real-time quantitative PCR assays to determine the transgene copy number in our new transgenic lines. Performed 8-color immunostaining for analysis of B-cell populations by flow cytometry via the FACSCanto instrument. Show less

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  AutoGenomics, Inc.

  Sept 2014 - Feb 2017

  Senior Research Associate

  The INFINITI® system is designed specifically for processing AutoGenomics'​ unique proprietary BioFilmChip® microarrays that can be multiplexed and configured with hundreds of biomarkers to assess disease signatures from a single sample template. As a member of the R&D Chemistry group, I...-executed research and development projects with a focus on biochemical and process improvements for molecular diagnostic applications, utilizing a variety of molecular biology techniques.-collaborated with other departments supporting the transfer of assays to the Clinical Laboratory.-co-managed the planning and execution of research and development studies.-performed experiments using advanced molecular biology techniques including multiplex PCR, quantitative PCR, variant genotyping, and sequence validation.-examined data and prepared technical reports, summaries, protocols, and manufacturing procedures. Show less

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  Bellicum Pharmaceuticals, Inc.

  Mar 2019 - Apr 2020

  Senior Assay Development Associate

  Performed assays for the validation of final cell therapy products utilizing the following methods:• Multiplex qPCR (Taqman based) assays to determine vector copy number.• Multiplex Flow Cytometry based assays (up to 10-color staining).• Measured Human IFN-gamma levels in cell culture supernates via solid phase ELISA.

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  MD Anderson Cancer Center

  Apr 2020 - May 2022

  Biologics Development Associate 3

  After acquisition of Bellicum assets (same workplace, same team members).Performed Quality Control release testing on therapeutics manufactured in the MDACC Cell Therapy Manufacturing Facility. Release testing assays include multiplex Flow Cytometry based assays (up to 10-color staining), Droplet Digital PCR and quantitative PCR based assays.

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  CTMC / A joint venture between Resilience and MD Anderson Cancer Center

  May 2022 - Sept 2023

  QC Specialist 3

  Following the Joint Venture formation with Resilience (same workplace, same team members).The Cell Therapy Manufacturing Center / A joint venture between Resilience and MD Anderson Cancer Center. Performs Quality Control release testing on therapeutics manufactured in the Cell Therapy Manufacturing Center.• Performs Edit Efficiency Assays on CRISPR edited eTIL products via ddPCR.• Performs Vector Copy Number analysis on CAR-T products via ddPCR and qPCR.• Performs Replication Competent Retrovirus (RCR) detection assays via qPCR.• Performs Phenotype analysis on CAR-T products via Flow Cytometry.• Facilitates tech transfer from other departments to integrate new assays for QC use.• Authors Standard Operating Procedures for new assays and instrumentation. Show less