Smitha Sreedharan

Experience

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  Institute of Bioinformatics (IOB)

  Jul 2003 - Jan 2004

  Research Assistant

  • Annotated protein for Human Protein Reference Database (www.hprd.org)

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  AUKBC Research centre

  Jan 2004 - Sept 2005

  Research Assistant

  • Studied dynamics of polymer transport across a membrane nanopore using optical tweezer

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  Uppsala University, Department of Neuroscience

  Jan 2006 - Nov 2006

  Project Student

  • Screened for genetic markers of the spinal lumbar locomotor central pattern generators• Studied developmental defects in EphA4/EphB2 KO mice• Gained experience in animal experimentation and immuno- histochemical techniques

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  Uppsala University, Department of Neuroscience, Unit of Functional Pharmacology

  Dec 2006 - Sept 2011

  PhD Scholar

  Functional characterization of centrally expressed Solute carriers and GPCRs• Generatedconditional knockout mice for SLCZ1• Setup transport assays in mammalian cells and Xenopus oocytes for orphan solute carriers• Trained Masters students in performing various experimental techniques• Optimized various behavioural paradigms in mouse model• Written and published research articles

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  Uppsala University, Department of Immunology, Genetics and Pathology

  Feb 2012 - Aug 2016

  Post doctoral fellow

  Investigating the influence of cell-of-origin on tumor development and phenotype of pediatric glioma• Developed mouse models for pediatric high grade glioma• Established several patient derived cancer stem cell cultures• Trained PhD students in performing various experimental techniques• Trained and mentored Master students in planning and execution of research thesis• In collaboration with pathologist developed a scheme for grading mouse brain tumor• Successful in writing and obtaining various research grants and travel grant. Show less

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  Institute of genomics and integrative biology (CSIR)

  Apr 2014 - Sept 2016

  Visiting Research Fellow

  Screening molecules that bind intracellular G-quadruplex DNA structure for anti-cancer effect in patient derived glioma stem cells.• Setup the cancer stem cell culturing lab• Established and validated the protocols for obtaining patient derived cancer stem cells• Trained and mentored PhD students in trouble-shooting research methodologies• Analyzed mouse models with high grade glioma• Setup and optimized cell based assays to test for oncogenicity

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  Indian Institute of Science Education and Research (IISER), Pune

  Apr 2017 - May 2018

  Scientist

  Exploring the epigenetic basis of early life stress, antidepressant therapy and neurogenesis on complex brain functions• Initiated the projects of COEE Phase II and established fundamental techniques required for the projects1. Setup the neuronal and neurosphere cell culture facility 2. Established animal experimentation procedures3. FACS sorting of neural stem cells from mouse hippocampus• Trained and mentored Postdocs and JRFs in the execution of the project• Conducted lectures for BS-MS students in Behavioural Epigenetics• Managed collaboration between IISER, Pune and TIFR, Mumbai• Processed samples for ChIP sequencing and RNA sequencing• Basic training of data analysis tools used in NGS analysis• FACS sorting of neural stem cells from mouse hippocampus• Written research grants and routine reporting of work updates/data to grant agencies• Collaborating to study anti-cancer effects of molecules that bind intracellular G-quadruplex in patient derived cancer stem cells Show less

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  Cactus Communications

  May 2018 - Oct 2018

  Editor
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  Indegene

  Oct 2018 - Apr 2020

  Scientific Writing Lead
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  IQVIA

  Apr 2020 - now

  • Associate Director (Engagement Manager)

    May 2022 - now

  • Senior Consultant

    Apr 2020 - Apr 2022