Experience

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  University of st andrews

  Oct 2007 - Sept 2008

  Marie-curie postdoctoral research fellow

  • Introduced various ligands in proteins and oligonucleotides to create artificial metalloenzymes and oligonucleotide-based hybrid catalysts (LC-MS, NMR, UV-Vis);

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  University of edinburgh

  Oct 2008 - Sept 2011

  Glaxosmithkline postdoctoral research fellow

  • Developed 3 new type of reactions which are compatible with automated chemical synthesis procedures (liquid handling, purification and analysis). • Improved practical, teamwork and interpersonal skills by doing research at GlaxoSmithKline’s Stevenage site (15 months).• Determined identity, stability and purity of drug-like molecules using UPLC, NMR, FT-IR and LC-MS.• Used electronic laboratory notebook to document research data and reaction procedures. • Presented research data at group meetings and international conferences. Show less

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  Institute of organic chemistry and biochemistry, academy of sciences of the czech republic

  Jan 2012 - Sept 2013

  Postdoctoral fellow

  ▪ Synthesized and analyzed highly elaborate functional helical aromatic compounds (up to 15 steps, GC-MS, FT-IR, NMR, chiral HPLC, ESI-MS)▪ Used computational chemistry for the elucidation of various reaction mechanisms• Gained hands-on experience handling air-sensitive compounds (glovebox and Schlenk techniques).• Presented research at group meetings and international conferences.

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  Friedrich-alexander-universität erlangen-nürnberg

  Sept 2013 - Jul 2016

  Postdoctoral research fellow

  - Synthesized and tested numerous singlet oxygen fluorescence sensors (small molecules, up to 7 steps synthesis).- Synthesized various oligos using K&A H-8 DNA/RNA synthesizer- Synthesized various phosphoramidites used for DNA solid phase synthesis- Modified nucleic acids with photosensitizers, fluorescent dyes, singlet oxygen-sensitive linkers. - Used HPLC, LC-MS, MALDI-TOF, NMR, UV-Vis, fluorimetry to analyze modified nucleic acids. - Studied kinetics of DNA/RNA templated reactions- Developed DNA-probes for detection of endoperoxides on the graphene oxide surface. Show less

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  Cytrx corporation / centurion biopharma corporation

  Aug 2016 - Nov 2018

  Scientist - drug discovery

  • Played an important role in the successful completion of pre-clinical development of 4 novel oncology prodrug candidates (small molecules).• Conducted identification, structure elucidation and quantitative determination of impurities and degradation products in prodrugs and pharmaceutical formulations (HPLC, LC-MSn, NMR).• Ensured efficient running of analytical instruments, i.e. maintenance, monitoring and calibration (LC-MS, HPLC, pH meter).• Chemically modified complex natural products to improve their therapeutic potential. • Supported the transfer of technical information to the contract manufacturing organization for clinical scale production of prodrugs.• Co-wrote 3 provisional patent applications and 3 papers.Skills:• Proficient in HPLC/UPLC (RP, SEC, HILIC, HIC, IEC, chiral) analysis with PDA/UV/ELSD detection.• Hands-on experience with analysis techniques such as LC-MSn, ESI-MS, GC-MS, MALDI, FT-IR, NMR (1D, 2D), UV-VIS, fluorimetry, titrations.• Experienced in developing and validating analytical methods.• Excellent practical knowledge of sample preparation methods for complicated matrices (solid phase extraction, analytical derivatization, protein precipitation, filtration, liquid-liquid extraction, Soxhlet extraction etc).• Solid experience in organic and organometallic synthesis (up to 15 linear steps synthesis, 100 µg – 100 g).• Catalysis by transition metals (Pd, Co, Ni, Cu, Rh, Ru, Fe, Au).• Profound knowledge of reaction mechanisms and retrosynthetic analysis.• Extensive experience with purification techniques, such as ion-exchange, reverse phase and normal phase chromatography (Biotage purification systems, LC-MS preparative systems).• Proficient in carrying out various reactions under pressure using pressure vessels / flow reactors / microwave reactors.• Practical experience in computational chemistry calculations (transition state, HOMO-LUMO gap, pKa, charge distribution, etc.). Show less

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  Spirochem

  Aug 2019 - now

  Team leader ii - research chemistry - manager of highly potent substance laboratory

  Engaged in synthesis, purification, characterization and delivery under tight deadlines of fine chemicals for drug discovery and development.