Kaitlyn Ng

Experience

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  Self Employed

  Jun 2020 - Jan 2023

  Private Tutor

  • Observed and evaluated students' performance towards academics• Enhanced students' learning by optimizing instructional approaches and using creative visualization of concepts• Revised lesson plans and formulated new ones to accommodate students' needs• Kept students focused with positive reinforcement strategies and encouragement

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  A*STAR - Agency for Science, Technology and Research

  May 2021 - Jul 2021

  Undergraduate Researcher (UROPS)

  Project Aim: Protein expression of Malassezia Secreted Aspartyl Proteases in Pichia Pastoris• Conducted protein expression experiments of Malassezia Secreted Aspartyl Proteases in P.Pastoris.• Ran PCR-based experiments, performed plasmid construction via gibson assembly, and conducted transformations of bacteria (E.coli and P.Pastoris).• Isolation of plasmid via Miniprep and Maxiprep• Analyzed substrate assays to measure protein expression, performed protein purification with Pepstatin-Agarose Resin, and identified proteins.• Acquired skills in PCR, SDS-PAGE, Gel Electrophoresis, Western blot, and various molecular techniques. Show less

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  National Parks Board

  May 2022 - Jul 2022

  Summer Intern

  • Set up and validate eDNA and metagenomics protocol for animal virus detection.• Data collected and contributed in released publibication (Yin, Chen, J et al.,(2023). Establishing environmental DNA and RNA protocols for the simultaneous detection of fish viruses from seawater. Environmental DNA. https://doi.org/10.1002/edn3.418)• Learnt virus concentration methods & Nucleic Acid extraction • Run qPCR/Real-time PCR for aquatic virus detection and quantification.• Assist in processing animal organs and environmental samples for aquatic virus detection Show less

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  Roquette

  Dec 2022 - May 2023

  Biopharma Intern

  • Handling upstream mammalian cell culture, cell metabolite analysis etc.• Downstream protein purification and analysis via analytical techniques such as FPLC, AIEX, HPLC- SEC• Formulation development and analysis of current modern vaccines• Characterization and analysis of nanoparticles using state-of-the-art equipment and fluorescence-based RNA – quantitation assay (Zetasizer, Unchained Lab’s Stunner, Quant-it™ RiboGreen RNA Assay Kit, Transfection assay)• Run qPCR/Real-time PCR for the detection and quantification of viral titer Show less

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  GenScript

  Aug 2023 - May 2024

  Protein Expression Associate Researcher

  • Conduct mammalian cell cultures (HEK and CHO cells) and related experiments (chemical transfection, electroporation).• High throughput screening of samples using techniques like SDS-PAGE and Western blot.• Adhere to SOPs for order production, and manage instruments used (Biosafety Cabinet, Nanodrop, etc.) by ensuring the equipment is well-maintained and in proper working conditions at all times, and that there is enough capacity to meet the production demand.• Perform data analysis and troubleshoot data related to daily operations and protein/antibody expression quality.• Support the production of cell-based therapy products in a GMP facility• Demonstrate understanding and compliance in Standard Operating Systems, Good Manufacturing Practice (GMP) and Good Documentation Practice (GDP).• Strictly follow aseptic techniques and practices per relevant SOPs (e.g. cell culture, cell recovery, cell cryopreservation, transfection, electroporation).• Carry out regular cleaning and upkeep of the equipment and classified rooms, as per relevant SOPs. Show less

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  ESCO ASTER PTE LTD

  Jun 2024 - now

  Biotechnologist