Sirine Benmamar

Experience

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  Centre de recherche en biotechnologie (CRBt)

  Jan 2021 - Sept 2021

  Chercheur stagiaire

  Optimization of the production and culture of protoplasts of two Solanum tuberosum varieties. Solanum tuberosum L. (potato) represents a crop of great importance, which can be improved by genetic engineering using protoplasts. Hence the objective of this study to optimize the concentrations of lytic enzymes of the protoplast isolation medium; cellulase and pectinase. First, micropropagation of vitroplants of the two varieties: Sarpo mira and Arizona was performed to preserve the plant material during the study. Then, different enzymatic combinations were tested for protoplast isolation. Finally, a verification of the viability of the protoplasts obtained from the previous step, considered as another objective, was conducted by initiating the culture of protoplasts on solid and liquid media but this attempt was not successful. These results open doors to many perspectives and improvements that would serve the objectives of a CRBt project of which this work has been a part. Voir moins

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  INRAE

  Jan 2022 - Jan 2022

  Chercheur stagiaire

  Internship in the INRAE unit "Institut Jean-Pierre Bourgin (IJPB)" involving a contribution to the functional analysis of orthologous genes of LEC1 in pea. Pea is a protein-rich field legume that requires little nitrogen fertilizer. Pea cultivation therefore offers undeniable economic and environmental advantages in the context of a growing need for protein. LEAFY COTYLEDON 1 (LEC1) is a major seed development transcription factor involved in the development and accumulation of seed lipid and protein reserves. In this study, we functionally compared four naturally occurring variants in pea with AtLEC1, which are phylogenetically related. The study is made through transient gene expression in the moss Physcomitrella patens. Voir moins

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  INRAE

  Jan 2023 - Jan 2023

  Chercheur stagiaire

  Internship at the INRAE unit "Biology and risk management in agriculture (BIOGER)" concerning the functional characterization of genetic determinants involved in atypical gene-for-gene interactions between oilseed rape and its fungal pathogen Leptosphaeria maculans.Oilseed rape (Brassica napus) is a crop primarily grown for animal and human consumption but also for biofuel. One of the major diseases responsible for crop loss in oilseed rape is stem canker caused by the fungus Leptosphaeria maculans. The main strategy employed against this pathogen is genetic control using specific resistance genes (Rlm). The Rlm proteins are capable of recognising effectors secreted by L. maculans (hereafter called avirlence / AvrLm proteins), whose main role is to facilitate infection and modulate host defence responses. Recognition of an AvrLm protein by a plant Rlm protein triggers plant resistance. Recent preliminary results revealed atypical infection phenotypes in some Rlm7/AvrLm4-7 interactions. To characterize the molecular determinants involved in these atypical gene-for-gene interactions, pathogenicity assays, expression kinetics analysis, genotyping at resistance locus and polymorphism analysis have been performed. Voir moins

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  INRAE

  Oct 2023 - now

  Doctoral Student

  PhD at the INRAE unit "Horticulture and seed research institute (IRHS)" concenring the characterisation of an effector from the fungal pathogen Venturia inequalis, causing apple scab.