John K. Muchena. Ph.D., PMP.

Experience

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  East Tennessee State University

  Aug 2007 - Jul 2009

  Teaching/Research Assistant

  Researched, initiated and managed an HPLC based method development for determination of the active components in hot pepper fruits.Developed and implemented quantitative HPLC separation method to determine the amount of capsaicin and dihydrocapsaicin at different seasons (summer, winter) and stages (green, yellow and red) of pepper fruit ripening using UV detection.

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  University of Georgia

  Aug 2009 - May 2014

  Research Assistant

  Developed innovative methods for structural determination of glycosaminoglycans (GAGs) using mass spectrometry, ion mobility and statistical techniques that enabled;(a) Challenging structural elucidation of highly sulfated GAGs such as pharmaceutical drug Arixtra.(b) Separation and characterization of isomeric GAGs that are otherwise difficult to separate using traditional LC and CE methods.(c) Principal component analysis (PCA) to differentiate and identify specific diagnostic ions from information rich EDD and CID spectra of isomeric oligosaccharides.Designed efficient method development for enzymatic protein digestion for FT-ICR analysis using ECD, CID and IRMPD that enabled sequence determination using web-based databases.Trained and guided graduate, undergraduate interns and visiting scholars on how to use, troubleshoot, and solve problems related to the operation of FT-ICR and how to analyze data.Successfully participated in interdisciplinary research collaborations with scientist at Complex Carbohydrate Research Center (University of Georgia, Athens, GA), Rensselaer Polytechnic Institute (Troy, NY) and Bruker Daltonics (Billerica, MA). Show less

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  University of California-Davis

  Jun 2014 - Aug 2017

  Postdoctoral Researcher

  ● Developed and validated methods LC-MS/MS methods for analyzing and quantifying peptides, proteins, glycoproteins and glycans for identifying human disease biomarkers for cancer, autoimmune and other diseases. Used complex biological samples like plasma, serum, milk, urine, tissues, etc● Designed a discovery method to identify high density lipoprotein (HDL) proteins site specific glycan heterogeneity using LC-MS/MS and multiple reaction monitoring (MRM).● Developed and executed protocols for monitoring glycosylation of recombinant plant generated therapeutic glycoproteins at different stages of bioengineering process.● Utilized and devised statistical methods using JMP and other methods, to compare disease and control cases using glycoproteomics data.● Collaborated with internal and external teams of scientists at UCD medical center, food science, engineering departments as well as Defense Threat Reduction Agency (DTRA). Managed and guided graduate, undergraduate researchers, and visiting scholars on their projects to ensure they were progressing effectively. Show less

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  Kerry

  Sept 2017 - Apr 2021

  ● Actively engaged in building and expanding RD&A LC-MS, LC-MS/MS, and preparative HPLC capability for high-throughput biomolecule characterization.● Collaborated with internal and external teams/stakeholders to develop and implement critical chemometrics and data processing tools allowing timely data guided decision making.● Conducted comprehensive research, designed, and implemented new LC-MS and LC-MS/MS workflows for proteomics and lipidomics applications focused on providing value to downstream product development.● Initiated and managed cross functional teams’ collaborative projects/discussions within North America and global teams to grow the taste innovation and analytical space in the company.● Lab scale to manufacturing scale new product development: Researched, initiated and scaled up 2 new taste modulation products.● Managing projects daily ensuring they are completed in a timely manner to facilitate product development and innovation that meet company targets.Patent co-inventor● Composition and methods for preparing hemicellulose-rich extract from spend coffee groundUS-2021017299-A1 Show less

  • Sr. Research Scientist - Taste & Nutrition Innovation

    Mar 2021 - Apr 2021

  • RD&A Research Scientist - Taste & Nutrition Innovation

    Sept 2017 - Mar 2021
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  Abbott

  Apr 2021 - now

  • Principal Scientist -Abbott Core Diagnostics

    Jun 2022 - now

  • Sr. Scientist- Abbott Core Diagnostics

    Apr 2021 - Jun 2022