Nicola Trim

Experience

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  Babraham Institute, Cambridge

  Jan 2007 - Jan 2008

  Student

  I was working on a project looking at the effect of over-expression of variant forms of alpha-synuclein on calcium signalling in human SH-SY5Y cells. I was responsible for generating and sustaining SH-SY5Y cells stably over-expressing variant forms of the protein alpha-synuclein and exploring its effect on calcium signalling pathways. I performed cell transfection through the use of JETPEI; monitored protein expression in my cells lines with western blotting, performed live cell imaging with FURA-2 AM dye, and experimented with TET operated gene expression in immortalised neuronal cell lines. Show less

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  Cambridge University Hospitals NHS Foundation Trust

  Oct 2008 - Dec 2012

  Trainee Clinical Scientist

  This position was a 4 year rotational clinical scientist training course in haematology. Throughout my rotational training I gained knowledge and experience which enabled me to perform routine and specialist diagnostic tests across all disciplines of diagnostic haematology. I undertook a number of research and development projects: 'the immunohistochemical detection of p53 overexpression in myelodysplastic syndrome with associated del(5q)' which I did in collaboration with the histopathology dept. and Professor Alan Warren at the University of Cambridge; 'Gene signatures in indolent mantle cell lymphomas' which I did in collaboration with the Professor Elliot Campo and the University of Barcelona ; 'heparin monitoring in heavily transfused patients' which i did in collaboration with Papworth Hospital cardiology department and Dr Martin Besser; and an in-house project looking at 'the detection of TP53 mutations in CLL patients'). These research projects enabled me to become contributing author on 4 peer reviewed publications. During my time in haematology, my preferred field was haemato-oncology, in particular genetics, so I decided to move into the field of genetics. Show less

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  Le Centre de Thérapie Cellulaire, Insitut Paoli-Calmettes

  Feb 2012 - May 2012

  Stagiaire

  In my final year of my training I took a 4 month unpaid, voluntary, secondment to work in Marseille, France in the Centre de Thérapie Cellulaire at the Institut Paoli-Calmettes to further my knowledge of stem cell and cord blood transplantation. I worked under the supervision of the Head of the JACIE committee, Professor Christian Chabannon to assess the viability of CD3+ cells in peripheral blood stem cells harvests after freeze-thaw processing.

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  West Midlands Regional Genetics Laboratory

  Jan 2013 - Apr 2017

  Involvement in processing of a large number of samples received within the haemato-oncology department of this regional genetics laboratory. Based within the myeloproliferative neoplasm team and bone marrow transplant monitoring with responsibilites to: manage the workflow; assign tests based on clinical referral reasons, clinical history and scientific knowledge; analyse and interpret test data; troubleshoot when require; and write reports. I analysed data obtained from various testing methods: RT-PCR; RQ-PCR; short tandem repeat analysis; droplet digitalPCR; Sanger sequencing; and G-band chromosome analysis. In addition to these duties, I also undertook duty scientist, scientific support, and Saturday working rota roles. Show less

  • Pre-registration Clinical Scientist

    May 2016 - Apr 2017

  • Research and Development Scientist

    Jan 2014 - May 2016

  • Pre-Registration Clinical Scientist

    Jan 2013 - Jan 2014
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  University Hospitals Birmingham NHS Foundation Trust

  Apr 2017 - Dec 2019

  Senior Clinical Scientist, Molecular Pathology

  Clinical Scientist in Molecular Pathology within the Clinical Pathology Services at the Queen Elizabeth Hospital, Birmingham. I am part of the Molecular Pathology team, providing genomic analysis of solid tumours with a specific focus on clinically-actionable genomic targets. As part of my role as a senior member of staff, I am involved in overseeing the genomic technical workflow, and the management and training of the technical team. I specialise in the technical set up of next generation sequencing and capillary sequencing and the analysis of this data, and the reporting of lung cancer EGFR testing and NGS analysis. Show less

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  Nottingham University Hospitals NHS Trust

  Dec 2019 - Aug 2021

  Lead Clinical Scientist, Molecular Diagnostics (Cancer)
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  AstraZeneca

  Sept 2021 - now

  Oncology Diagnostics Manager