Experience

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  Cnrs - centre national de la recherche scientifique

  Jan 2015 - Jan 2015

  Engineering assistant internship

  • INFINITY lab in Toulouse, formerly UDEAR "Epidermal Differentiation and Rheumatoid Autoimmunity Unit" (UMR 5165) • Principal investigator : Dr Léonor NOGUEIRA• Subject : “Cross-reactivity analysis of rheumatoid arthritis specific antibodies populations through cross-inhibition experiments and comparative analysis of their diagnostic performance" • keywords : rheumatoid arthritis - autoimmune diseases - ELISA - statistics

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  Inserm

  Sept 2015 - Jan 2016

  Engineering assistant internship

  • Subject : "Development and characterisation of a 3D lymphoma cell culture system"• Keywords : 3D cell culture - preclinical models - cancerology - multicellular spheroids - alginate• Principal investigators : Dr Laurence Bresson BEPOLDIN• Collaboration with Dr Pierre NASSOY from Institut Optique d’Aquitaine CNRS UMR 5298

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  University health network

  Jan 2016 - Jan 2016

  Engineering assistant internship - toronto general research institute (ontario, canada)

  • Subject : "Role of cardiac macrophages in tissue injury and repair following ischemic injury and viral myocarditis"• Keywords : cardiac - macrophages - tissue repair - viral myocarditis• Principal Investigator : Dr Slava EPELMAN

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  Poietis

  Jan 2017 - Jan 2017

  Final year internship

  • Subject : "Optimization of a dermis biofabrication process by 3D printing"• Keywords : 3D bioprinting - collagen - fibroblast - keratinocytes• Supervisors : Dr Delphine Fayol and Marine Salducci

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  Cirimat

  Oct 2017 - Sept 2020

  Phd student

  I have conducted my PhD at the crossroad of material sciences and cellular and molecular engineering, at CIRIMAT lab and I2MC lab (Toulouse, France). My PhD project was about 3D alginate-chitosan biomaterials for soft tissue cell therapy. In practice this work has included the study and validation of alginate/chitosan scaffolds sterilization by ionizing radiation on the one hand, and the proof of concept of those 3D matrices in the field of cellular therapy for chronic wound healing on the other hand.General skills : project management • supervision of a 3 research interns/students • communication (oral and written) • data analysis • autonomy • multidisciplinarity • proactivity • adaptability • bibliographical watch • interpersonal skills Technical skills : bone marrow-derived macrophages and mesenchymal stromal cells isolation (mouse and rat), cell culture techniques, cell-based assays including cell viability assays or macrophages polarization, molecular biology (RNA isolation, RT-qPCR), immunolabelling (-histochemistry, -fluorescence, scaffold cryosections, histological skin sections, image acquisition by confocal/bi-photon microcope and analysis, biopolymer-based hydrogel/scaffolds formulation, cast-freezing, supercritical CO2 drying, material physico-chemical characterization (scanning electron microscopy, size exclusion chromatograpy, rheology, texture analysis, x-ray tomography, thermal analysis), low energy electron-beam ionization, understanding of the regulatory context (Eur. Pharmacopoiea, International standards e.g. ISO 11137-2)Industrial collaboration with ITHPP Alcen (Thégra, France)Supervisors : Pr Sophie Girod Fullana (CIRIMAT lab) and Pr Brigitte Sallerin (I2MC lab) Voir moins

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  Université paul sabatier toulouse iii

  Oct 2017 - Sept 2019

  Teaching assistant in galenics

  • Teaching experience : assistant for galenic practical works at Faculté des sciences pharmaceutiques (Toulouse, France)• Management experience : supervision of tutored projects

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  Restore

  Oct 2020 - Jan 2022

  Postdoctoral research fellow

  Preclinical evaluation of 3D biopolymeric scaffold for cell therapy of chronic wounds / Patent drafting for ATMP developmentGeneral skills : project coordination • supervision of a 6-month engineer • intellectual property (patent writing) • communication (oral and written) • data analysis Technical skills : use and protection of laboratory animals (designer level diploma), cell-based assays including cell viability assays or macrophages polarization, molecular biology (RNA isolation, RT-qPCR), selection of biopolymer raw materials, biopolymer-based hydrogel/scaffolds formulation, material physico-chemical characterizationDeliverable : brevet FR2302374 “Matrice 3D poreuse à base de complexes de polyélectrolytes et ses applications” Principal investigators Dr. Beatrice Cousin & Dr. Agnès Coste Voir moins

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  Medxcell sa

  Jan 2022 - Jan 2024

  Scientific project manager

  Development of a new advanced therapy medicinal product (ATMP) based on human mesenchymal stromal cellsGeneral skills : project management • supervision of a R&D engineer and a R&D technician • communication (oral and written) • data analysis • protocol development • results-oriented • rigor • proactivity • flexibility • scientific and technical watch • GLP and GMP-like envrironmentTechnical skills : adipose derived-mesenchymal stromal cells expansion in bioreactor (hollow fiber) • cells transfection • development of immune cell-based functional tests • immune cells isolation and culture (PBMCs, lymphocytes, monocytes, dendritic cells) • flow cytometry • cytokine quantification by ELLA technology • ADCC • NK cells mediated cytotoxicity • Voir moins