Indira Akula

Experience

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  Drexel University

  Jan 2001 - Jan 2001

  Research Assistant Intern

  Laboratory of Dr. Thomas Edlind • Worked with 96 well assays, plasmid isolation of yeast cells and SDS gels• Performed invitro drug testing, aseptic and molecular genetics techniques• Analyzed data, interpreted results and searched literature• Compiled full report of experiments, which was reviewed by lab and college internship coordinators and earned seven academic credits and “A” grade

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  University of Pennsylvania

  Jun 2002 - Apr 2005

  Research Technician

  Laboratory of Dr. Robert Doms:• Performed RT-PCR,PCR, ELISA, DNA cloning, mini, mega and maxi preps, Site directed Mutagenesis, DNA sequencing analysis, Immunoblotting and Immunoprecipitation• Tissue culture with adherent and suspension cell lines, growing Hybridomas and purifying Antibodies• Maintained 293T, Vero, BHK-21, Hela, 3T3, Cos-7 and Cho cell lines• Expressed Proteins in bacteria as a fusion with thioredoxin with aHistidine tag and purified using Histidine tag• Contributed to work published in Virology 336 (2005) 70–82 – “Characterization of neutralizing antibodies to West Nile virus”.Laboratory of Dr. Paul Bates: • Performed vector cloning, expression, protein production, purification, SDS-PAGE gels, Western Blots, PCR, tissue culture and DNA sequencing analysis• Transfected mammalian cell lines, fluorescent microscopy, virus production, purification and viral infectivity assays• Conducted chemical cross-linking, pull-down and biochemical assays• Prepared materials for Immunogold Electron Microscopy• Assisted Post-Doctoral Fellows in FLOW Cytometry• Subcloned, expression and purification of GST- Fusion proteins using Pharmacia FPLC and Bio-Rad Duo Flow chromatography systems • Performed general lab duties including lab organization, ordering reagents, radiation safety monitoring, allocations of charges to various grants and record keeping Show less

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  Mount Sinai Hospital, Toronto

  Jan 2010 - Oct 2014

  Research Technician

  • Conducted molecular cell biology studies and high throughput biology by using LUMIER assays to look at protein-protein interaction• Experienced in establishing stable cell lines using various transfection methods like calcium phosphate, lipofectamine and nucleofection.• Proficient with standard molecular biology techniques ( DNA cloning, PCR, RNA extraction and analysis, Western blotting, Immunoprecipitation and Immunoflorescence• Expertise in preparation of competent cells for bacterial work and casting protein gels for running Western Blots• Organizing -80°F freezer, maintainance and allocating space for lab members• Efficient in ordering lab supplies, interacting with vendors, attending product shows and research safety meetings and receiving lab supplies• Working as a volunteer for Scihigh program to promote curiosity for science in elementary schools by co-ordinating scientist in the classroom visits Show less

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  The Hospital for Sick Children

  Oct 2014 - Mar 2016

  Laboratory Research Project Coordinator
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  University of Toronto

  Apr 2017 - Dec 2019

  Research Technician
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  Specific Biologics

  Feb 2022 - Apr 2022

  R&D Laboratory Technician
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  Ozmosis Research Inc.

  Mar 2024 - now

  Clinical Data Manager