Deeksha Shetty

Experience

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  Bhabha Atomic Research Centre

  Mar 2014 - Sept 2014

  Research And Development Intern

  1. Purified histidine-tagged CRP protein using a combination of Low-Pressure Liquid Chromatography and Affinity Chromatography to obtain high-quality protein for functional assays.2. Investigated the interaction of CRP protein with its proposed binding site in the upstream region of cspD in E. coli using Electrophoretic Mobility Shift Assay (EMSA), providing insights into regulatory mechanisms.3. Determined CRP interaction with the CRP binding site in vivo by constructing a cspD-gfp fusion clone, which included a deletion of the CRP target site in the cspD promoter region, to study regulatory effects.4. Standardized an in vitro transcription assay to reveal CRP-mediated regulation of cspD, contributing to a deeper understanding of bacterial toxin regulation. Show less

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  Tata Consultancy Services

  Jul 2014 - Dec 2015

  Regulatory Affairs Associate

  1. Conducted quality checks on regulatory documents, including New Drug Applications (NDA), Annual Reports, and Development Safety Update Reports (DSUR) to ensure compliance with industry standards.2. Reviewed essential study documents such as study protocols, informed consent forms, investigator brochures, investigational new drug annual reports, and clinical study reports, ensuring accuracy and adherence to regulatory guidelines3. Managed relationships with key clients, including GlaxoSmithKline (GSK), and contributed to enhancing the quality and consistency of clinical reports for regulatory submissions.4. Led the TCS Quality Control team within the Medical Writing Department, providing training on internal and external quality control standards for regulatory submission documents. Show less

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  University of Saskatchewan

  Jan 2016 - Jan 2019

  Graduate Research And Teaching Assistant

  1. Executed wet-lab procedures for next-generation sequencing (NGS), including DNA/RNA isolation, library preparation, and operation of the Illumina Sequencing Platform.2. Analyzed large NGS datasets using the R platform, covering Whole Genome Sequencing (WGS), RNA-Seq, and 16S sequencing to derive meaningful biological insights.3. Developed and implemented customized bioinformatics pipelines for the analysis of sequenced data, utilizing tools such as IGV, Bowtie, STAR, and QIIME to ensure robust data processing.4. Achieved high accuracy in sequence alignment, quality control, differential gene expression, and pathway enrichment analysis using Gene Ontology (GO), KEGG, and Reactome.5. Employed statistical methodologies, including multiple hypothesis correction to calculate false discovery rates and implemented linear models using tools like Limma, DESeq, and edgeR for comprehensive data interpretatio6. Recognized for outstanding research efforts, receiving the award for Best Master's Thesis and winning a prestigious global competition, highlighting the impact of my work in the field. Show less

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  McGill University - Faculty of Science

  Jan 2019 - Oct 2019

  Research Assistant - Bioinformatics

  1. Assisted in analyzing 18S rRNA gene amplicon sequencing data to characterize eukaryotic communities, utilizing bioinformatics pipelines such as Mothur, network analysis (MENA pipeline), and Cytoscape, along with assessments of alpha and beta diversity. Contributed to two peer-reviewed publications that enhanced the understanding of microbial communities.2. Collaborated on data analysis and visualization efforts using Python and R, leveraging packages such as SciPy, NumPy, Matplotlib, Pandas, ggplot2, and dplyr to effectively manipulate and represent complex data.3. Assisted the principal investigator in grant writing and producing results for impactful scientific publications, contributing to the broader scientific community.4. Conducted protein characterization and purification by identifying gene distributions and employing techniques such as nucleic acid extraction, PCR, real-time PCR and Western blotting.5. Established and maintained ciliate cell cultures, utilizing advanced methodologies including flow cytometry, qPCR, EMSA, and sequencing to investigate microbial persistence and pathogen transmission.6. Conducted network analysis using bioinformatic pipelines and tools to elucidate the relationships and interactions within microbial ecosystems. Show less

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  Genecis Bioindustries Inc.

  Oct 2019 - Jan 2025

  Scientist - Molecular Microbiologist and Bioinformatic Specialist

  (Promoted from Scientist II to Scientist V and below are key highlights of my work)1. Directed bioinformatics workflows and led R&D activities in microbiology, bioinformatics, and fermentation, focusing on optimizing strain selection and enhancing performance.2. Trained, hired and mentored 15+ team members on Benchling, utilizing Python, SQL, and data visualization techniques to automate workflows, increasing sample processing capacity by 50%.3. Played a key role in leading the NGEN AI project, leveraging the potential of digital twin technology to transform model predictive scale-up and technology transfer processes.4. Contributed in technology transfer activities, prepared documentation to transition processes from the benchtop to pilot scale and then to the Contract Manufacturing Organization (CMO).5. Secured over $2M in research grants for bioprocess and genomics projects, while managing 3+ external client collaborations, leading to a 20% increase in revenue.6. Spearheaded projects on mixed microbial cultures, delivering pivotal data that secured Series A funding and provided key technical insights during investor discussions.7. Led the design and execution of Design of Experiments (DOE) for mixed microbial cultures, driving the development of innovative fermentation processes and improving yield and efficiency.8. Contributed in automating genome analysis pipelines using Nextflow, accelerating biomanufacturing advancements and improving workflow scalability.9. Developed, modified, updated, and implemented Standard Operating Procedures (SOPs). Led recruitment efforts, providing training and coaching for new hires. Show less

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  University of Toronto

  Jan 2020 - Dec 2020

  Sessional Instructor

  1. Led the design and development of lesson plans and presentations for courses in molecular biology and bioinformatics, incorporating cutting-edge tools and methodologies.2. Accountable for managing student teams in bioinformatics projects, evaluating assignments, and conducting assessments to ensure a comprehensive understanding of data analysis techniques.3. Managed laboratory supplies and maintained records , including data collection and preprocessing, while assisting in the preparation of experimental materials and protocols. Show less