Andras Saftics, Ph.D.

Experience

• 

  Research Center for Natural Sciences, Hungarian Academy of Sciences

  Jan 2012 - Aug 2014

  • Master Thesis Student

    Feb 2012 - Aug 2014

  • Bachelor Thesis

    Jan 2012 - Jun 2012
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  Centre for Energy Research

  Sept 2014 - Jul 2020

  - Developing MATLAB-based software packages for biosensor data analysis (optical waveguide lightmode spectroscopy, OWLS; quartz crystal microbalance, QCM), including the kinetic analysis of adsorption and affinity interactions (https://nanobiosensorics.com/nbs-software).- Analyzing the affinity binding kinetics of lignan-based small molecule therapeutics and ions to proteins using the high sensitivity grating coupled interferometry biosensor (GCI – an SPR analog technique).- Optimizing antibody immobilization chemistries on polymer-based substrates for bacteria detection in urine samples. Show less - Developing surface chemistries for the fabrication of dextran-based hydrogel nanolayers applied as low-fouling functional coatings on label-free biosensor surfaces. Optimization of hydrogel crosslinking and spin-coating methods.- Characterization of thin dextran films using ellipsometry, atomic force microscopy (AFM), attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FTIR), x-ray photoelectron spectroscopy (XPS), and contact angle goniometry (CAG). Investigating the nanostructure and hydration properties of hydrogel layers by parallel optical (OWLS) and acoustic (QCM) methods.- Developing cell micropatterning methods using robotic fluidic force microscopy for cell adhesion studies; studying cell adhesion using the high-throughput resonant waveguide grating biosensor (RWG) and digital holographic microscopy (DHM).- Developing surface chemistries on gold surfaces for cyclic voltammetry-based nickel detection in natural water sources. Show less

  • Research Fellow

    Apr 2019 - Jul 2020

  • Assistant Research Fellow / Ph.D. Student

    Sept 2014 - Apr 2019
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  City of Hope

  Aug 2020 - Mar 2025

  Postdoctoral Fellow

  - Developing single-molecule localization microscopy (SMLM) and total internal reflection fluorescence microscopy (TIRFM) based extracellular vesicle (EV) assays and related surface chemistries for biomarker discovery.- Developing image analysis and data processing codes in MATLAB.- Optimizing imaging methods on a Nikon Ti2 NSTORM super-resolution microscope. Optimizing bioconjugation methods for labeling proteins and extracellular vesicles with fluorescent dyes as well as immobilization of antibodies and DNA/RNA aptamers on solid supports. Developing SMLM-based immunohistochemistry (IHC) methods for cancer-related biomarker detection in tissues.- Isolation of EVs from human blood plasma using size exclusion chromatography (SEC). Characterization of EVs using nanoparticle tracking analysis (NTA), single-particle interferometric reflectance imaging sensor (SP-IRIS), transmission electron microscopy (TEM), and Western blot (SDS-PAGE) assays. Show less

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  Countable Labs

  Nov 2024 - now

  Senior Engineer, Image and Data Analysis