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Neeraja Kulkarni, PhD

Neeraja Kulkarni, PhD

Research Trainee

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location of Neeraja Kulkarni, PhDSingapore

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  • Timeline

  • Skills

    Western blotting
    Elisa
    Hplc
    Sds page
    Flow cytometry
    Immunology
    Immunofluorescence
    Luciferase assay
    Molecular cloning
    Fluorescence microscopy
    T cells
    Bone marrow chimera
    Pcr
    Biochemistry
    Gel electrophoresis
    Dna
    Molecular biology
    Cell culture
    Microbiology
    Biotechnology
    Life sciences
    Protein purification
    Antibodies

  • About me

    Holds Singapore dependent pass and Currently working on effect of Chemokine receptor CCR6 on plasticity and function of Treg and Th17 cells during ulcerative colitis. Publications are as follows https://www.ncbi.nlm.nih.gov/pubmed/?term=neeraja+kulkarni Few more publications are on the way!!

  • Education

    • National centre for cell science

      2012 - 2017
      PhD Immunology

    • Ramnarian Ruia College, Matunga (University of Mumbai)

      2008 - 2010
      Master of Science (M.Sc.) Biotechnology

      Activities and Societies: Participation in ‘Paradigm inter collegiate quiz competition’ organized by Biotechnology department of G.N.Khalasa college, Mumbai in 2008. Participation as a coordinator in organizing intercollegiate biotechnology event ‘Chimera 2008’ at Ramnarain Ruia college in 2008.

    • Birla College of Arts, Science & Commerce, Kalyan (University of Mumbai)

      2005 - 2008
      Bachelor of Science (B.Sc.) Biotechnology

      Activities and Societies: Participation in conferences and Bioinformatics workshop in ‘Biozeal 2007’ organized by Biotechnology department of Thadomal Shahani Engineering College in 2007 Participation in events of ‘Galaziao 2006 inter collegiate biotechnology program’ held by G.N.Khalsa College in 2006 Training at Immunology department of ‘Jaslok Hospital and Research Centre’, Mumbai in 2006 Hands on training in “Plasma Diagnostic Laboratories and Blood Bank Component Research Centre” for 15 days in 2006

  • Experience

    • Serum Institute of India Pvt. Ltd.

      Jun 2009 - Sept 2009
      Research Trainee

      Worked on a Project entitled, 'Method development for erythropoietin dimer formation and its estimation by size exclusion HPLC'.Utilized various techniques like size exclusion HPLC, UV spectroscopy, polyacrylamide gel Electrophoresis, isoelectric focusing, silver staining, dialysis.

    • NII - National Institute of Immunology

      Sept 2010 - Dec 2010
      Research Trainee

      Worked on projects entitled,'The Study of Single nucleotide polymorphism in PTPN22 gene by RFLP analysis and its role in rheumatoid arthritis' & 'Study of Diagnostic potential of anti-cyclic citrulinated peptide (anti-CCP) antibodies in Rheumatoid Arthritis'Utilized many techniques like PCR, RFLP, ELISA, western blot, DNA RNA and serum isolation from blood Biochemical analysis of blood and serum samples etc.

    • Indian Institute of Technology, Bombay

      Dec 2010 - Sept 2011
      Research Scholar

      I was studing the DNA packaging machinery in the family of largest known viruses called as mimivirus and mamavarius.

    • National center for cell sciences, Pune

      Jul 2012 - Jul 2017
      Doctoral Researcher

      Brief about my PhD work, it was to understand the role of chemokine receptor CCR6 in the differentiation and plasticity of CD4 T cells during gut inflammation. With the help of different in vivo colitis models, clinical samples from ulcerative patients, and in vitro differentiation assays, we established that CCR6 promotes the differentiation of pathogenic Th17 cells and suppress Treg differentiation and function during colitisI have hands on experience in using flowcytometry, Immunohistochemitry, real time PCR and ChIP.List of my publications: https://www.ncbi.nlm.nih.gov/pubmed/?term=neeraja+kulkarni Show less

    • ImmunoScape

      Nov 2017 - now

      • Team Lead (Data Analysis)

        Jan 2022 - now

      • Research Scientist

        Mar 2019 - Dec 2021

      • Associate Research Scientist

        Nov 2017 - Feb 2019

    • Inflection Point Ventures

      Sept 2021 - now
      Angel Investor

  • Licenses & Certifications

    • BD FACS sorting

      BD
      Mar 2018

    • Certified user of BD Canto FACS

      BD