Experience

• 

  Antoni van leeuwenhoek

  Apr 2004 - Dec 2005

  Laboratory technician

  My task in this project was analysis mutations by using Real-time PCR, allelic discrimination (SNP Genotyping Assays, point mutations), and Genes scan for DSDI: (Detection of Small Deletion and Insertion).

• 

  Uniqure

  Jan 2006 - Mar 2012

  Assistant research scientist

  Characterization of the therapeutic gene using the molecular biological and biochemical approaching adeno associated viral vectors (rAAV) for gene therapy treatment of rare inherited genetic disorders. The rAAV batches with therapeutic genes using the baculovirus system for the proof of concept, preclinical, and safety in vitro and in vivo studies.* Development and validation of recombinant adeno associated viral vector-based gene therapy of lipo lipase enzyme deficiency (Glybera), haemophilia, and Huntington’s disease.* Characterization of the therapeutic gene using the molecular biological and biochemical approach* Develop potential AAV-vector serotype* Generation of recombinant AAV using different vectors* Impurity study drug substance (rAAV finale product)* Monomeric duplex of transgene* Develop UniQure baculovirus backbone and patented cell culture, stable cell line generation Show less

• 

  Baseclear bv

  Apr 2012 - May 2015

  Senior research technician

  Together with my colleagues, I was involved in different projects for:* custom cloning, PCR cloning, cloning of variant libraries (Site Saturated libraries & Combinatorial libraries),* Mutagenesis.* Next gene sequencing and sanger method.* I focused on seamless cloning technology, and I performed a comparative study on these. It was an achievement for the company.

• 

  The janssen pharmaceutical companies of johnson & johnson

  May 2015 - Jul 2018

  Associate research scientist

  I worked with a team of scientists responsible for driving innovation in biological products e.g., a vaccine, Antibodies.My responsibilities consisted of cloning and developing bioassay methodes for the characteristic of vaccines produced with adenovirus in the patented PER.C6 cells* Technology transfer.* Adenovirus, AAV and HIV vector generation* Vaccine backbone (Heavy and Light chain)* Development of droplet digital PCR * Data analysis and communication results.* I worked with scientists to develop problem-solving strategies. Show less

• 

  Dsm

  Jul 2018 - Nov 2020

  Senior research development technician

  As Sr Technician Genetics. I performed and contributed to research projects to guarantee the best results at the lowest costs. * Design of interest genes, pathways using Bio-informatics and cloning tools like the golden gate, Gibson assembly. Genome engineering * Genome Editing of strains of bacterial species using an Engineered CRISPR /Cas9 system method.* Maintained a clean and organized working environment to enhance productivity.

• 

  Eurofins

  Nov 2020 - now

  Consultant at jansen biologics

  I helped the Covid-19 vaccine team at Janssen Biologics by focusing on quality control testing. * Performed accurately and precisely routine testing of the in-process, finished product * IDPCR, QPA, and VPQPCR test methods * Escalating quality issues ( out of spec) and manage deviations* Conduct quality investigations using Event Trackwise System (ETS)* Tracking corrective and preventive actions (CAPA)* Qualified Train the Trainer ( TTT) on the job Johnson & Johnson.* Collect, and store experimental data sets and document analytical results to ensure maximum traceability.* Trend data analysis * Prepare data overviews and reports, including interpretation of results in the Elims.* Ensure all laboratory activities are performed according to applicable protocols.* Strong Knowledge in Tech Transfer methods and Gap analysis.* Training junior team members and coaching the students * Ensuring the proper implementation and development of new research technologies Show less