Karissa Choy

Experience

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  SASE - UC Santa Cruz

  Sept 2016 - Jun 2017

  Science Committee

  •Engaged in a discussion and talked with students about the Keck Institute for Graduate studies leaving with an idea of the various pathways after graduation•Communicated with the president about lab tours and mentoring sessions

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  University of California, Santa Cruz

  Sept 2017 - Oct 2018

  Undergraduate Research Assistant - Chen lab

  Biochemistry department.• Working under a Grad student researching about fuel cells for a greener footprint.• Learned how to ask questions that made me intrigued.• Formed a mentor/mentee relationship where I could ask them why they were interested in their research.

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  Galileo Learning

  Jun 2018 - Aug 2018

  Internship

  • Worked with groups of students to make up rules for a mystery game • Communicated with parents on what their kids were working on• Led day care and checked in students • Called parents about lost and found items • Filed papers and prepared student lunches

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  Santa Cruz Women in Business

  Sept 2018 - Apr 2019

  Member

  •Forming connections of how to become a leader •Create a mentor/mentee relationship through Linked-in •Talk with other members about lack of women in tech

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  Accent International Study Abroad

  Jun 2019 - Aug 2019

  UCEAP Made in Florence

  •exposed myself in a different country, which included communication barriers, navigating transportation, culture differences, different school system with oral and discussion based classroom setting and testing•interviewed bakery owner by asking what they ate and what groceries they bought in Italian at an open air market•learning the history of Florence by walking through different sites, such as the Duomo •understanding how to buy items in a farmers market and how to ask for clothes at a market by walking around and figuring out which Italian words correspond to the english word Show less

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  University of California, Santa Cruz

  Sept 2019 - Jun 2020

  •Project based on deleting several genes in Vibrio Cholera. •Performed homologous recombination by using an E.coli vector. •Followed by selecting for the Vibrio cholera plasmid by using Rifampicin in petri dishes that were cultured with the media from the donor and recipient. •Used sucrose to select for Vibrio cholera plasmid by finding the plasmids that were 1kb long. •Incorporated Lux reporter and biosensor into mutated Vibrio genes•Measured the luminescence and fluorescence of mutated gene incorporated with Lux reporter and biosensor Show less

  • Undergraduate Student Researcher - Yildiz Lab

    Sept 2019 - Jun 2020

  • Senior Design project - Saltikov lab

    Sept 2019 - Jun 2020
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  Chan Zuckerberg Biohub

  Jun 2020 - Aug 2020

  Computational Biologist Intern

  •Project based on comparing two sequencing pipelines that comprise of information from the human and mouse genome•Used 10x and SplitBio to run the pipelines, Cell Ranger and Split-seq, to compare the total cells, barcode rank plot, mean reads to genes, mean reads to UMIs, sequence coverage, barnyard experiment plot•Ran single cell sequence pipelines by creating a reference genome, which is then run along with fastQ files from the human and mouse •Parse and read through a fastQ file by using jupyter notebook to gain a sense of how many cells were sequenced •Extracted data from CSV, bam, html files to visualize data by creating algorithms from scratch•Used Scanpy, bamnostic, to analyze data output files from the Illumina sequencer•Comparing between 10x and SplitBio methods to discern the tradeoff as 10x sequences fewer cells and SplitBio has a lower multiplet rate•Presented Journal Club paper on mapping differentiation pathway of pluripotent stem cells with use of scRNA-seq•Presented the comparison of two single cell sequencing pipeline in the internal seminar Show less

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  AccuraGen

  Jan 2021 - Jun 2023

  Research Associate

  •Focus on optimizing early screening, target selection, MRD assays.•Handle different sample types from FFPE, cfDNA, miRNA.•Create Illumina and MGI libraries from extraction to sequencing.•QC with Qubit, Tapestation, Bioanaylzer.

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  Carnegie Mellon University

  Jan 2024 - May 2024

  Bioseparation and Bioprocess Analytical Techniques

  •Perform Dialysis on mAbs to then run through zeta sizer to get zeta potential and size of mAb•Run several mAbs through CE to characterize size of peaks, analyze elution time, and concentration

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  Boehringer Ingelheim

  Jun 2024 - Aug 2024

  Analytical Science intern

  •Compared 3 Next Generation CE-SDS instruments: •Revitty Lab Chip, Agilent Proteoanalyzer, Biotechne Maurice•Streamlined pipette process to accommodate low intensity of peak signal •Optimized analysis, particularly peak integration and normalization method•Focused on generating Std. curve by looking at accuracy, linearity, and precision

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  Carnegie Mellon University

  Aug 2024 - Dec 2024

  •Work with three other group members to create 2 machine learning algorithms (Random forest and logistic regression), focusing on the prediction of asthma, by performing pre-processing, processing, and analysis. •Obtained asthma dataset from kaggle data site for use in algorithm •Performed Analysis on the two algorithms by focusing on confusion matrix and AUC curve •Implement Emerald Cloud lab to perform error prone PCR in creating diverse condensates for directed evolution •Troubleshooted 'no PCR signal' by re-running PCR and editing the code •Communicated with ECL personnel to understand how to perform deeper analysis, such as looking into the performance of the PCR machine

  • Machine Learning- Prediction of Asthma project

    Aug 2024 - Dec 2024

  • Emerald Cloud Lab- Synthetic bio Project

    Aug 2024 - Dec 2024