Akhila Ajith

Experience

• 

  Central Tuber Crops Research Institute (CTCRI)

  Jun 2017 - Jun 2017

  Student Intern

  * Agarose gel electrophoresis * Aseptic Handling * Pipetting * Streak Plate Technique * Tissue Culture Techniques

• 

  Rajiv Gandhi Centre For Biotechnology (RGCB)

  Jul 2019 - Aug 2019

  Student Intern

  Undergone training in Molecular Biology and Tissue Culture techniques * Aseptic Handling * Pipetting * Streak Plate Technique * Mock & Actual Infection * RNA Isolation * Tissue Culture Techniques * Agarose gel electrophoresis *PCR

• 

  Speridian Technologies

  Oct 2020 - Jul 2021

  Data Validation Trainee
• 

  Biozeen, Bangalore Biotech Pvt Labs Limited

  Sept 2021 - Oct 2021

  Internship Trainee

  *Aseptic Handling *Sterilization Techniques *Handling BHK 21 cell line *Principles of Subculturing*Media formulation *CIP & SIP *Cell Counting *Growth Curve Analysis *Karyotyping *Cryopreservation *Functional Design of Bioreactor *Serum Studies *Buffer Preparation *Fermentation with Pichia pastoris *Parameter optimization

• 

  DD Enterprises

  Apr 2022 - Oct 2023

  • Understanding and reviewing User Requirement Specifications (URS)• URS compliance and preparation for Process Flow diagram as per URS system• Designing and Preparation of Techno- commercial offers, Process Flow Diagrams (PFD) and Piping and Instrumentation Diagrams (P&IDs) for Bioreactors, Fermenters, Process vessels, Filtration Skids and CIP and SIP system as per URS.• Ensuring ASME BPE and US FDA (21 CFR Part 11) regulatory standards are implemented in the Design• Drafting 2D P&IDs and PFDs of skid from draftsmen for the designed systems• Actively involving with inter departments for the effective execution of the project• Process Calculation on Utility consumption• Focusing on engineering, feasibility, and Safety development of new technology• Discussing requirements with vendors, arranging, and following up for offers from vendors required for client commercial offer preparation• Preparation of Bill of Material (BOM) for the proposals Show less

  • Bioprocess Engineer

    May 2023 - Oct 2023

  • Bioprocess Engineer trainee

    Apr 2022 - May 2023
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  NNE

  Nov 2023 - now

  Mechanical Engineer