Leona Wagner

Experience

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  Probiodrug AG

  Oct 1998 - Aug 2012

  Research Assistant,

  2011 - 2012: Project leader: Project 4 (Alzheimer disease)• Proteolytic degradation of several Abeta-variants and their respective fibrils by different peptidases 2008 - 2012: Collaborator: Side-project (Huntington Disease)• Degradomics of Neuropeptide Y (NPY) in CSF from patients with Huntington Disease to evaluate NPY-RIA. 2005 - 2010: Project leader: Project 3 (stress-related diseases)• Identification of dipeptidyl peptidase 4 (DPP4) as a novel pharmaceutical target for stress- related indications 2000 - 2004: Research scientist: Project 2 (type-2 diabetes) • Characterisation of DPP4 and DPP4-like enzymes in islets of Langerhans 1998 - 1999: Research scientist: Project 1 (type-2 diabetes) • Purification of porcine DPP4 from kidney cortex and isolation of its isoforms to improve crystallisation Methods:- Tissue-, cell- & organelle-extraction- Protein purification & characterisation - Assay development (enzymatic activities & inhibition studies)- Sample preparation (MALDI-TOF-MS & LC-MS/MS)- Neuropeptide/peptide stability in serum, blood & CSF by MALDI-TOF-MSExpertise:Proteases: Dipeptidyl peptidases 4, 8, 9 & 2, fibroblast activation protein alpha, meprin A & B, cathepsins D, B, G, L & S, plasma kallikrein, tissue kallikrein, ACE, ACE2, IDE, thimet oligopeptidase, neurolysin, urokinase, plasmin, trypsin, thrombin, MMP's 2, 3, 9 & 13, NEP, NEP2 & prolyl endopeptidase.Neuropeptides/Peptides:Proteolytical processing of: NPY, Galanin, Substance P, GALP, PACAP, Orexin A+B, beta-endorphin, endomorphin 1+2, ACTH, CRH, oxytocin, Arg-vasopressin, neurotensin, dynorphin A, Abeta, Abeta-fibrils, ghrelin, GLP-1, GIP, PYY + angiotensin 1. Neuroendocrine:Investigated the influence of proteases in the neuroendocrine system + its implications in several pathological conditions, focusing particularly on the so-called circumventricular organs such as median eminence and area postrema. Show less

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  MSBM

  Jan 2011 - Jan 2014

  Participant at 5th, 7th and 8th Mass Spectrometry in Biotechnology & Medicine (MSBM)

  Summerschool of mass spectrometry for medical and life science students

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  Deutschsprachige Selbsthilfegruppe für Alkaptonurie (DSAKU) e.V.

  Feb 2012 - Aug 2021

  Chairman, patient representative (volunteer work)

  - Dissemination of Alkaptonuria (AKU) to patients, their families and medical professionals- Developing of strategies on disease managements for AKU-patients to improve quality of life- Raising awareness of AKU and rare diseases- Project Management of patient-support group- Network-building- National and international presentations

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  University of Mainz

  Nov 2013 - Jan 2014

  Advanced Training in LC-MS/MS and LC-MRM assays

  - Assay-Development- Stress-Molecules- Pain-Molecules

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  Mibeg Institute Medicine

  Oct 2015 - Jun 2016

  Advanced training of Clinical Data Manager

  - Clinical Data Management- MedDRA- Pharmacovigilence- Medical terminology- Pharmacology- Medical Registry- ICH-GCP/AMG- Excel/Access- CDISC- PHP/MySQL- VBA- SQL- SAS/Biometry

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  Medical University of Innsbruck

  Mar 2016 - Jun 2016

  Advanced Training Clinical Data Manager

  - MedDRA-Coding- Literature-research- Data Management / Medical Information Systems /Databases- Rheumatology- Rare Diseases

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  Medical University of Innsbruck

  Jan 2017 - Apr 2017

  Project Team Member

  Clinical Data Manager- MedDRA-Coding- LOINC-Coding- Clinical Data Management

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  Joint Federal Committee (Gemeinsamer Bundesausschuss, G-BA)

  Sept 2021 - Jun 2022

  Scientific Advisor at the Department of Methods Assessment and Prescribed Services
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  Joint Federal Committee (G-BA)

  Jul 2022 - Nov 2022

  Scientific Advisor for Patient Involvement at the Innovation Committee (Innovationsausschuss)