Denise Stern

Denise Stern

Senior Scientist

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location of Denise SternMetro Jacksonville

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  • Timeline

  • About me

    Laboratory Manager at City of Jacksonville

  • Education

    • University of West Georgia

      -

      Previously known as West Georgia College

    • University of North Florida

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      Bachelor of Arts (B.A.) Psychology Summa Cum Laude
    • The University of Georgia

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      Bachelor of Science (B.S.) Microbiology
    • Florida State College--Jacksonville

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      Associate of Science - AS Biotechnology Laboratory Technology
    • The University of Georgia

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      Master of Science (M.S.) Microbiology

      *Taught Undergraduate Laboratory classes as graduate level Teaching Assistant: Introductory Microbiology (4 classes), Lab Methods in Microbiology Williams, D.K., and S.A. Martin. 1990. Xylose uptake by the ruminal bacterium Selenomonas ruminantium. Appl. Environ. Microbiol. 56: 1683-1688.Williams, D.K., and S.A. Martin. 1989. Transport of xylose by the ruminal bacterium Selenomonas ruminantium. 89th Annual Meeting of the American Society for Microbiology, May 14-18, New… Show more *Taught Undergraduate Laboratory classes as graduate level Teaching Assistant: Introductory Microbiology (4 classes), Lab Methods in Microbiology Williams, D.K., and S.A. Martin. 1990. Xylose uptake by the ruminal bacterium Selenomonas ruminantium. Appl. Environ. Microbiol. 56: 1683-1688.Williams, D.K., and S.A. Martin. 1989. Transport of xylose by the ruminal bacterium Selenomonas ruminantium. 89th Annual Meeting of the American Society for Microbiology, May 14-18, New Orleans, LA. Abstract: K 82 Show less

  • Experience

    • Betz PaperChem [Betz Dearborne, Hercules, Ashland]

      Jan 1989 - Jan 1994
      Senior Scientist

      *Initiated research to investigate the chemical interferences of biocides.*Worked as part of a team to develop high thru-put bacteriological assays to screen potential efficacious actives.*Completed project to control anaerobic odor-causing bacteria in paper mills; planned and directed the construction of an oxygen-scrubbing column and gassing manifold.*Completed independent project to develop a colorimetric assay to evaluate the efficacy of actives and biocides over time using a tetrazolium compound and a scanning spectrophotometer.*Completed project to screen the efficacy of biocides with several bacterial species using an isotope uptake assay. Show less

    • Duval County School Board

      Jan 1995 - Jan 1996
      Substitute Teacher

      *Temporarily substituted for teachers in the public school system.

    • Maxwell House- via AccuTech

      Jan 1996 - Jan 1997
      Quality Control Technician

      Evaluated quality of industrial food product (coffee): weight checks, incidental substances, foreign particles, moisture, density, color, caffeine, and flavor; containers checked for code dates, lithography, and appearance; data entry; measured caffeine levels with HPLC and aroma oils with GC.

    • Vistakon (Johnson & Johnson)- via King Company/King Temporary Staffing

      Jan 1999 - Jan 2000
      Microbiologist

      Worked as part of a team to develop innovative sterilization method and assisted with cell adhesion experiments. Identified bacteria using VITEK and API; enumerated bacteria with serial dilutions and plate counts; initiated, directed, and authored research which led to a procedural change in a primary assay.

    • Florida Department of Health- Environmental Laboratory Certification Program

      Jan 2001 - Jan 2011

      *Audited ~300 environmental laboratories for compliance with the National Environmental Laboratory Accreditation Program (NELAP) Standards [now TNI] [ISO 17025]. *Responsible for auditing, writing reports, and reviewing/approving corrective actions for cited deficiencies. *Updated AAMS database.*Presented at Florida Society of Environmental Analysts (FSEA), "2003 NELAC Standards Microbiology Checklist, How to Pass Your Assessment"​http://www.floridahealth.gov/licensing-and-regulation/environmental-laboratories/environmental-laboratory-certification/index.html Show less

      • Environmental Laboratory Assessor (Biologist III/Chemist III)

        Jan 2004 - Jan 2011
      • Biologist II

        Jan 2001 - Jan 2004
    • Florida State College at Jacksonville

      Jan 2001 - Jan 2004
      Adjunct Professor

      *Taught Undergraduate Biology and Laboratory classes BSC 2010C: Principles of Biology BSC 1005: Life in Its Biological Environment BSC 1005L: Biology Laboratory

    • Environmental Conservation Laboratories, Inc.

      Jan 2011 - Feb 2015
      Quality Assurance Manager

      *Managed quality assurance program at an environmental laboratory (chemistry and microbiology); responsibilities included data review/audits, proficiency testing, reviewing/updating standard operating procedures [ETQ], root cause/ corrective action, compliance with regulatory requirements [FL DOH-NELAP/TNI; NC DHEC, DoD], hosting external regulatory audits and associated responses, writing monthly QA reports, updating LIMS [Element] database, performing thermometer calibrations.http://www.encolabs.com/ Show less

    • City of Jacksonville

      Oct 2015 - now

      Perform microbiological and chemical analyses for a NELAP/TNI accredited environmental laboratory relevant to river and ocean water quality. Proficient in SM 9222D Fecal coliform membrane filtration method; Quanti-Tray analyses for Total Coliform/E. coli and Enterococci.Quality Assurance Officer. NELAP/TNI Technical Director- MicrobiologyRevised Quality Assurance Manual, Wrote SOP for Quanti-Tray methods. Independently performed crucial work toward Quanti-Tray application to the Florida Department of Health/TNI/NELAP for certification.Performed air sampling relevant for EPA TO-15 analyses. Show less

      • Laboratory Manager

        Aug 2019 - now
      • Chemist

        Oct 2015 - now
  • Licenses & Certifications

    • Biotechnology Specialist - Technical Certificate

      Florida State College at Jacksonville
      Apr 2016
    • Biotechnology Laboratory Specialist - Technical Certificate

      Florida State College at Jacksonville
      Aug 2016