Marie Buczek

Experience

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  Institute for Molecular Biology, University of Aarhus

  Jan 2004 - Jan 2007

  Stud.scient

  Research in the role of the enzyme DNA topoisomerase II in transcription using yeast (S. Cerevisiae) as a model organism.

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  FCMB ApS

  Dec 2007 - Aug 2011

  Molecular Biologist

  Development of a method for risk-free prenatal diagnostics by isolating and analyzing the genome of fetal cells from maternal blood.Main-responsible for the projects:• Gene expression profiles of fetal cells isolated from maternal blood. This project involved Laser capture microdissection of cells using laser microscopes at Carl Zeiss in Munich as well as microarray data processing • Isolation of individual fetal cells: Collaboration project with an Italian company, Silicon Biosystems, in Bologna (including several working-visits in their laboratories in Bologna)Other projects I was working on:• Development and testing of different purification methods for sorting out fetal cells from maternal blood• Test of antibodies for specific staining of fetal cells• Gender determination of fetuses by running real time qPCR on purified free fetal DNA from blood Show less

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  University of Southern Denmark

  Feb 2012 - Aug 2012

  Researcher

  I worked on a research project concerning investigation of the expression of microRNAs in colorectal cancer. I set up a method in the laboratory to measure the amount of certain microRNAs in blood samples by using quantitative PCR.

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  LEGO Group

  Dec 2012 - Nov 2014

  Product Safety Assistant

  Employed as Chemical Approvals assistant in the Chemical Approvals team in the LEGO Corporate Quality department. I supported the approval of materials used in production including approval of test reports from chemical test laboratories as well as managing declarations and other documentation. I was involved in developing and supporting the chemical approval process for Merchandise&Promotions products in collaboration with colleagues in the quality department in Shenzhen, China. Another key work task for me was management and cleaning of databases as well as moving data from our existing databases into SAP. I also worked with LEAN tools for optimizing work procedures and minimizing waste. Show less

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  Eurofins

  Jan 2014 - Jan 2015

  Molecular Biologist

  Employed as a Molecular Biologist at Eurofins Steins in the Serology department (maternity cover). My primary task was to approve results from real time PCR tests, performed to test for certain pathogenic bacteria in milk and food products. My most important task was to make sure that the production of test results was running smooth, to support the lab technicians in their work and to make sure that results were delivered to our customers on time and in the right quality.

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  Sotio

  Jan 2016 - Sept 2018

  Specialist in Process Development

  SOTIO is a Czech biotechnological company based in Prague, which is developing new cancer medicines based on active cellular immunotherapy. At SOTIO, I learned to work under GMP conditions. My work was primarily about improving existing QC tests, plan and conduct validations and to develop new molecular tests. In this position, I authored numerous detailed laboratory protocols and documented the outcome of experiments in scientific reports.

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  Bavarian Nordic

  Jul 2020 - Jun 2023

  Bavarian Nordic produces vaccines, among others a vaccine against Mpox• Developed, improved and validated ELISA assays, e.g. a potency test for a new Covid-19 vaccine developed under extreme time pressure. Also performed routine ELISA assays under GMP.• Performed FACS based titration of pox vectored vaccines (under GMP), a 3 day process including cell culture work with mammalian cells.• Responsible for general daily maintenance of laboratory equipment and qualification of new equipment (main responsible person for all cell culture incubators).• Wrote SOPs and scientific reports for method development.• Responsible for general lab-organization and training of new employees in the ELISA lab.• Initiated and followed through on change requests. Participated in writing deviations and found strategies for prevention of re-occurrence. Show less

  • Research Associate

    Apr 2022 - Jun 2023

  • Pharmacy Technician, QC

    Jul 2020 - Mar 2022
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  FUJIFILM Cellular Dynamics, Inc

  Mar 2024 - now

  QC Analyst III