Amruta Salunkhe

Experience

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  Om Greentech Industries

  Jul 2020 - Dec 2020

  Junior Research Scientist
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  New York University

  Sept 2021 - Feb 2023

  Course Assistant | Department of Biomolecular Sciences (BMS)

  - Assisted in undergraduate and graduate-level lab courses, including Genetics and Genomics and Advanced Cell and Molecular Biology. - Guided students in conducting experiments, setting up lab protocols, and troubleshooting procedures, ensuring a clear understanding of complex concepts. - Graded exams, quizzes, and lab reports, maintaining consistency and fairness in evaluation.

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  New York University - College of Dentistry

  Jan 2022 - Dec 2022

  Graduate Student Researcher | Witek Lab

  -Conducted research under the mentorship of Dr. Lukasz Witek, focusing on 3D printing bioceramic scaffolds (e.g., β-Tricalcium Phosphate, Alumina Toughened Zirconia) and their characterization for biomedical applications.-Evaluated the mechanical properties of scaffolds through compression testing to assess their suitability for load-bearing biomedical implant applications.-Performed in vitro studies to assess biocompatibility, including cell viability (PrestoBlue assay), osteoprogenitor cell attachment (fluorescence microscopy), and osteogenic differentiation (immunostaining).-Investigated guided bone regeneration using ex vivo tissue processing of dog and sheep mandibles, assessing implant integration and host tissue response.-Developed expertise in histological techniques, including sample embedding, microtome sectioning, slide preparation, immunohistochemistry, and histomorphometric analysis using the Aperio ScanScope.-Maintained primary osteoprogenitor cell cultures through routine passaging and media changes to ensure cell viability and experimental consistency.-Collaborated with interdisciplinary teams, including biomaterials scientists and postdoctoral researchers, to advance scaffold optimization for clinical translation. Show less

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  FirstLight Bio, Inc

  Jun 2022 - Aug 2022

  Gene Editing, Discovery Research Intern

  - Successfully created a cell line harboring a disease-specific point mutation, enabling the study of pathological mechanisms and therapeutic interventions.- Designed and executed experiments to optimize homologous recombination (HR)-based editing, in immortalized cell lines at therapeutically relevant loci.

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  Enceladus Bio

  Feb 2023 - May 2023

  Discovery Research Intern

  -Attempted to create a diseased cell line with large repeat expansions, leveraging advanced genetic engineering techniques to model disease-relevant conditions.-Conducted advanced genetic editing studies in human iPSC-derived neurons as part of collaborative projects with a major pharmaceutical partner, driving the development of innovative therapeutic strategies.

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  Enceladus Bio

  Jul 2023 - now

  Research Associate II

  - Conducted proof of concept studies for genetic liver disorders using iPSC-derived and primary human cell models.- A key member of the translational biology team driving drug discovery through an innovative all-RNA gene editing platform.- Designed, validated, and implemented in vitro assays for novel therapeutics.- Collaborated with cross-functional teams, including RNA synthesis and lipid nanoparticle (LNP) formulation specialists, to validate cutting-edge gene editing workflows.