Zachary Wolk

Experience

• 

  Miramar Beach Restaurant

  May 2008 - Sept 2008

  Busboy
• 

  4d Power

  Jun 2010 - Jul 2010

  Intern

  Assembled test equipment for measurement of biofuel properties.Measured conductivity and resistivity of glycerol solutions to support biofuel research.

• 

  College Works Painting

  Jun 2011 - Jul 2011

  Painter

  Exterior house painting and door-to-door marketing to find potential customers.Monitored on-site workers to ensure safety protocols surrounding lead paint and worker safety were followed.

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  Genentech

  Jun 2012 - Aug 2012

  ORDP Intern

  Performed purification studies with both chromatography and tangential flow filtration systemsLearned to program and operate chromatography skid software on UnicornExperience with HPLC analysis and ÄKTA chromatography machinesHelped develop a unit storage tracking system and populated the initial database

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  Genentech

  Jan 2015 - Apr 2017

  BioProcess Technician

  Purification technician in manufacturingTrained in GMP, GDP, sterile gowning and equipment transferQualified to operate in large scale manufacturing on:Tangential Flow Filtration, Chromatography, and Bulk FiltrationTFF cartridge assembly/disassemblyPool additions and titrationsAseptic Sampling and Visual InspectionBuffer preparation and batch recordsCleaning and Sterilization of equipmentEquipment changeover cleaning between products Experience in training and qualifying new hires on operationsInvolved in packing and unpacking of both fixed bed and hydraulic chromatography columns in sizes from 100L to 500LExperience with SAP inventory management softwareMember of DS Production Electronics Team since 12/2015, managing computers and other electronics within the group and being the point of contact with IT/technical support Show less

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  BioMarin Pharmaceutical Inc.

  May 2017 - now

  Clinical and Commercial cGMP manufacturing including Bacterial Fermentation, Centrifugation, Purification (Chromatography, TFF, and Filtration), PEGylation, and Formulation.Technical writing for SOPs and Work Instructions

  • Process Engineer II

    Sept 2021 - now

  • Technical Lead, Manufacturing

    Mar 2020 - Sept 2021

  • Sr. Manufacturing Associate

    May 2017 - Mar 2020