Ray C.

Experience

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  Hawaii Agricultural Research Center

  Jun 2012 - Mar 2013

  Laboratory Associate

  ● Participated in a BSL2 environment for micropropagation of sugar cane and apple bananas.● Cultivated plant cell tissue cultures for tropical plant research, met production goals and deadlines for shipping.● Management of samples; analyzed for bacterial/fungal contamination.● Assisted in lab operations including: media preparation, glassware cleaning, and autoclaving of tools/waste.

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  Novartis

  May 2013 - Oct 2013

  Laboratory Technician

  ● Processing, pick-up, and delivery of glassware and reagents, and providing quality support to all customers within Norvartis site.● Followed SLPs and maintained documentation, accurately recording data and data entry to GMP standards● Used and maintained laboratory equipment daily, and ensured sterility of glassware and reagents by using aseptic techniques or autoclaving procedures● Worked in a fast paced production environment that demanded a high level of multitasking and flexibility● Provided accurate work output data to supervisors, assisted with tracking of goals and production. Show less

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  Beryllium

  Nov 2013 - Mar 2015

  Research Associate II, Fermentation

  ● Executed laboratory activities to support contract and grant research.● Small scale and large scale fermentation of microbes.● Collection and maintenance of analytical data.● Responsible for SDS-PAGE, protein expression testing, Western Blotting, and related molecular biology activities● Preparation and sterilization of media and stock solutions.● Clean and maintain all Fermentation/Molecular Biology equipment, supplies, and reagents.

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  Seattle Children's

  May 2015 - Aug 2016

  Research Assistant, Bench (Jensen Lab)

  ● Responsible for overseeing the day-to-day operational needs of the laboratory.● Assisted in Molecular Biology core: Molecular Cloning, PCR, Gel Electrophoresis● Helped with Lentivirus Production, Concentration, Titering and Flow Cytometry Analysis

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  Fred Hutch

  Aug 2016 - Dec 2018

  Research Technician II, Molecular/Cellular Biology (Kiem Lab)

  ● Assisted in gene-editing projects relating to expression of CD33 for Acute myeloid leukemia (AML) and bcl11a gene therapy for hemoglobinopathies.● Various molecular/cellular biology responsibilities: cloning/ligations, nucleic acid purification, CRISPR in-vivo and in-vitro gene-editing electroporation/data analysis, ELISA, mouse & NHP blood/tissue processing, CD34+ Enrichments, CFU assays● Tracking of retroviral integration sites in cell populations using Next-Generation Sequencing technology. ◦ Preparing DNA samples for Illumina platform sequencing through a range of molecular biology techniques: PCR, DNA isolation, qPCR, Tapestation● Large scale production of retroviruses (foamy virus and lentivirus) for gene therapy/gene editing studies. ◦ Planned and executed viral vector production and titration ◾ Discussed vector design optimization ideas and concepts ◦ Transduction assays with readout by basic flow cytometry and qPCR (Taqman). Show less

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  Silverback Therapeutics

  Dec 2018 - Jul 2022

  Senior Research Associate II, Protein Engineering (Cell Sciences)

  ● Contributed to the discovery, validation, and development of cancer immunotherapeutics.● Responsible for production of recombinant proteins for new antibody therapeutics and reagents with transient transfection and with high producing stable cell lines.● Assisted in engineering research cell lines for in-vivo animal study and in-vitro cell-based assays.

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  Lyell Immunopharma

  Aug 2022 - Nov 2023

  Research Associate II

  ● Generated/maintained various cell line models and developed/optimized cell line based functional assays.● Assisted with setting up functional assays (sequential kill/serial stims) looking at killing (cytotoxicity by IncuCyte) and cytokine secretion (MSD) for both TIL and PBMC based products.● Ran a range of flow panels for TIL phenotyping during production and functional assays. (Attune NxT/Cytek Aurora)● Contributed to molecular biology work when needed (DNA extractions/ddPCR/cloning etc.) Show less