Anjana Munoz-de-Leon

Experience

• 

  Parc Taulí Hospital Universitari

  Oct 2012 - Feb 2013

  Laboratory Technician

  ◦ Document management, material classification, preparation of bioreactives, Maintenance of stock◦ Use of specialized software, SPSS, exel, acces, dna purity Nanodrop, for good analyse of samples◦ Hands-on experience on Lymphocyte, bone marrow and placental cell culture, dna extraction and staining chromosomal and cariotipatge◦ Profient using of fluorescence and confocal microscope analysed by Metapher, electrophoresis and PCR. Sequenciador ABI applied byosystems 3130 and genemapper.◦ Studies of patients with Prader Willi and Angelman, using UBE3A gene molecular study, MPLA and cases of mental retardation in adults using MS-MLPA and microsatellite analysis of chromosome 15 test.◦ Experience of writing, updating and maintaining departmental protocols and procedure Show less

• 

  Middlesex University

  Feb 2014 - Aug 2014

  Reaseach Assistant

  Tissue culture precedures: know viability of cell, thraw, keep the proliferation and differentiation in vitro of mesenchymal stem cells and differentiation in vitro of MSC’s, Hela, C33A, C2335, hepatocysts, keratinocysts and MCF7 cells. Grown cells in flask, coverslips and suspension, and take care of the different passages. Analyse the metabolism activity with AlarmBlue, counting cells under confocal microscope, keeping always a safe and sterile condition. Use Flow citometry procedure to analyze the early and late apoptosis with liposomes drugs addition. Add nanogold particle to skin cell to know the penetrance effect.◦ Histological procedures:liver, placenta, bone and skin tissue cutting with microtome, embedding and reembeding and mounting sample. IHC staining by superficial membrane markers to demostrate the presence of antibodies and Haematoxylin/Eosin staining.◦ Genetics procedures: agar gel electrophoresis and DNA sequenciation.◦ Prepare different agents, apply antibodies, score the result under microscope and cytometric graphics and western blot with a good labelling and tidiness. ◦ Teaching task: supervise and mark exams, introduce marks in student records, assist students in laboratory.◦ Supervise waste drump. Show less

• 

  Kingston University - Company

  Oct 2016 - Oct 2017

  Master Cancer Biology

  Project: “Silencing of FGFR1 on Caski cervical cell lines”- Tissue culture: Grow and maintain Caski cell line as well as check the viability by trypan-blue. Perform immunocytochemistry on cell receptors and ligands taking pictures of the slices under the EVOS microscope. Carry out wound healing and proliferation assays to assess the changes of growth on the cell, after FGFR1 siRNA transfection, analysed on IncuCyte Zoom software.- Molecular procedures: Proceed Western-blot protein levels using the Li-Cor analyser as well RT-PCR on Bio-Rad analyser.- Safety procedures: wastage the biohazard adequately, cleaning the laboratory, labelling all the chemicals and reagents. Show less

• 

  LGC

  Jan 2017 - May 2017

  Cell Culture Cancer Researcher

  Project “Assess the toxicity of DMSO in the cytotoxic effect of KHYG-1”- Tissue culture: Grow and maintain HepG2 and KHYG-1 cells as well as a passage, aliquot, thaw and feed cells in flasks. Cryopreservation in liquid nitrogen and Mister Frosty. Cheek cell Morphology using microscope and morphology Trypan-blue test on 96 well plates. Cell counting by the automatic ViX-cell software. Be aware of the cell viability, by CT-RES analyser and presto-blue test. Analyse data obtained from the different test and make graphs by Excel. - Laboratory support: Prepare different reagents (DMSO at different concentrations, bottles of media with FBS, aliquoted IL2) as well as make new protocols and assist in ordering.- Safety procedures: wastage the biohazard adequately, cleaning the laboratory, cheek fridges and freezer temperatures. Show less

• 

  IQVIA

  Mar 2021 - Aug 2023

  Be able to gate efficiently the results of the test in the software diva, to give the sponsor an accurateresults.◦ Obtain more knowledge of the FACSCanto and AURORA machines as well as trobleshooting ofcompensation, standarization and perform CD8 fluorochrome set up◦ Perform basic training to the new co-workers in the lab, showing them how to structure their day, how to obtain the information for the procedures, helping to understand and perform the SOP's.◦ Help the coordinator to organize the day, prioritise test to perform, calculate timings of the assay to upload into the machines in the best order to maximize machine usage.- green project leader, making the laboratory more ecosustenible Show less Perform routine analysis of patient specimens of blood and bone marrow samples, according to standardoperating procedures by staining with fluorescence antibodies of cell surface proteins, intracellular Ki andFOX proteins, as well as Receptor occupancy test. These assays are performed in the flow cytometerBDFAC CANTO and analysed with the software FACS diva. Moreover, able to use the lyse wash assistant(LWA) and Sample prepare Assistant (SPA) machines to faster the preparation test process.◦ Analyse the results of the test, Evaluate the validity of specimen results against the algorithms displayedby the lab computer system FACS diva, using gatting guides to assure the accuracy of the results, as wellas having the knowledge of immunology and cancer biology to asses the test.◦ Set up and shout down the flow cytometers CANTO and AURORA, following all the steps required givenin the SOP.◦ Be capable of performing machine troubleshooting, when it stops check the filters, change the reagents, beaware of the laser status.◦ Knowing the different quality control specimens (streck, Qlyo, JARk..) and how to use them in thedifferent tests.◦ Keep track of the work performed through Logs, be aware of the repeat sheet and know the collectiondates of the specimens to know if it's within stability to perform the assay required, maintain the machineslogs update to date as well as the fridges and freezers temperatures.◦ Perform pending workload review periodically during the day; find pending assays and resolveoutstanding work prior to the completion of testing for the day. Show less

  • Sicienfitic Associate

    Nov 2021 - Aug 2023

  • Medical Laboratory Technician

    Mar 2021 - Nov 2021
• 

  Charles River Laboratories

  Aug 2023 - Aug 2024

  Scientific Associate

  - Green team leader, make awareness of the green options.- Development, validation, and implementation of immunoassays - Processing of samples for GLP and non-GLP studies.-Recording and reporting of data according to the ALCOA principle.

• 

  University of Cambridge

  Aug 2024 - now

  Flow Cytometry Technician

  Organized the flow cytometry facility booking system, PPMS, to ensure optimal equipment availability and coordinate between users to improved experimental efficiency. Set up, calibrated and maintained the analysers (Cytoflex S, Cytoflex LX, Aurora Cytek, Attune NxT) and cell sorters (Aria II and, Discovery S8). Including daily start-up and shutdown procedures and any troubleshot. Handle and assist to prepare CL1 and CL2 samples including blood, lung, stem cells. Revise and improve the facility SOP including more environmentally friendly initiatives. Running panels ranging from two up to 50 colour and compensation and unmixing panels in standard and spectral flow for analysers and sorters. Trained users on machine operations, software usage and data analysis to support accurate and efficient experimental workflows. Basic knowledge of FlowJo and OMIP for data analysis. Assisted in panel design for research projects, tailoring configurations to meet specific experimental requirements Maintained laboratory cleanliness, managed waste disposal, organized supplies, tracked reagent inventory, and prepared PBS solution bottles. Show less