Marion Ferren

Experience

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  INSERM-U758

  May 2012 - Sept 2012

  Research Assistant (Internship)

  • Functional analysis of the measles virus with mutation on the polymerase complex• Execution of many virology experiments: viral production (paramyxovirus), viral titration (in TCID50/mL)• Good knowledge of molecular biology and biochemistry : DNA and RNA extraction, PCR, RT-qPCR Cloning, vector constructions, primers’design, Western blot• Acquisition of competences in cellular biology: cell transfections (Vero, HeLa, HuH7)• Oral presentations of my results in intern and extern meetings Show less

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  CIRI-INSERM-U1111

  Jun 2013 - Sept 2013

  Research Assistant (Internship)

  • Molecular and functional interactions of innate immunity receptors of the RIG-I family• Molecular biology and biochemistry : DNA and RNA extraction, PCR, RT-qPCR Cloning, vector constructions, primers’design, Western blot• Acquisition of competences in cellular biology: cell transfections (293T, HuH7), luminescence• Oral presentations of my results in intern and extern meetings .

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  CGphiMC - CNRS - Université Claude Bernard

  Sept 2013 - Jun 2014

  Research Engineer (intership)

  • Study of the formation of « DNA-contaning PML-NBs » during infection by the herpes simplex virus type 1.• Accomplishment of many Immunofluorescent staining and Fluorescent In Situ Hybridization (FISH).• Fluorescent microscop analysis.• Aquisition of virology competences : Infection of primary human fibroblatic cells (BJ), viral titration of non-replicative viruses (in Pfu)• Protocols drafting

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  Transgene

  Sept 2014 - Oct 2015

  Research Engineer (internship)

  • Focused on the developpment of TG1050, a targeted immunotherapy candidate for the treatment of chronic hepatitis B.• Provided immunological results after PEGylated adenovirus injection on mice: ELISPOT and flow cytometry (on mice splenocytes), neutralizing antibody titration (on mice serum)• Animal experiments on mice: spleen sampling, blood sampling, intraperitoneal injections, subcutaneous and intranasal injections.• English and french oral presentations for project meetings.

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  Fondation pour l'Université de Lyon

  Sept 2014 - May 2015

  Competitive exam Campus Création

  Entrepreneurship competitive examTeam workProject managementBusiness planhttp://www.campus-creation.fr/

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  University College Cork, Ireland (UCC)

  Dec 2015 - Mar 2016

  Research Assistant

  School of Pharmacy• Developpment of Sphingosine-1-Phosphate detection by HPLC• Detection of bEnd3 cells BDNF release by ELISA• Supervisory of two students

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  Columbia University Medical Center

  Jun 2016 - May 2018

  Staff Associate

  Department pediatrics and infectious diseases.Team Anne Moscona et Matteo Porotto.Responsible for research in several infectious diseases, specifically focused on mechanisms of entry and membrane fusion mediated by measles and parainfluenza virus.• Virology, virus production and virus titration• Reverse genetic system• Cellular biology, transfection, cell assays• Western Blot, Immunoprecipitation, Immunostaining• Implementation of a protocol to produce 3D Lung Bud Organoids from human embryonic stem cells (implantation of the method in the laboratory)• Infection of human airway epithelium and lung bud organoids• Training of students• Report writing and oral presentations Show less

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  CIRI Centre International de Recherche en Infectiologie

  Jun 2018 - Dec 2021

  Team NeuroInvasion, TROpism and VIRal Encephalitis (NITROVIRE)PhD mentor : Cyrille Mathieu.Physiopathology of central nervous system infection with respiratory viruses.

  • PhD Student

    Dec 2018 - Dec 2021

  • Engineer

    Jun 2018 - Dec 2018
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  ERASMUS MC

  Mar 2022 - May 2023

  Postdoctoral researcher

  Department of Viroscience. Team Rory de Vries and Rik de Swart. EMBO Postdoctoral Fellowship ALTF-977-2021

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  ESTBB - Ecole Supérieure de Biologie - Biochimie - Biotechnologies

  May 2023 - Sept 2023

  Enseignant chercheur

  Pathogenesis of chronic hepatitis B infection and liver cancer.Research group 7 directed by Philip Lawrence: Biosciences, Technologies and Ethics.

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  Inserm

  Jan 2024 - now

  Research engineer