Deep Bhattarai

Scientific Initiation Internship (Grade: 17/20 from the panel )

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Rijswijk, South Holland, Netherlands

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Timeline
About me
Biochemical engineer (AMIChemE)
Education
- UCL
  2022 - 2023
  Postgraduate Degree Biochemical Engineering
  My master's thesis includes"Chemical lysis and neutralization in an in-line static mixer for plasmid DNAs extraction."- Characterized in-line static mixer reactor.- Performed rheological study in the cell lysate fluid.- Successfully optimized the process and parameters for higher yield.- Conducted quantitative and qualitative studies on the extracted plasmid DNAs.Other modules that I extensively studied include as following,i. Advanced Bioreactor… Show more My master's thesis includes"Chemical lysis and neutralization in an in-line static mixer for plasmid DNAs extraction."- Characterized in-line static mixer reactor.- Performed rheological study in the cell lysate fluid.- Successfully optimized the process and parameters for higher yield.- Conducted quantitative and qualitative studies on the extracted plasmid DNAs.Other modules that I extensively studied include as following,i. Advanced Bioreactor Engineeringii. Integrated Downstream Processingiii. Bioprocess Systems Engineeringiv. Bioprocess Validation and Quality Controlv. Biochemical Engineering Laboratoryvi. Sustainable Industrial Bioprocesses and BiorefineriesVii. Vaccine BioprocessingViii. Fundamental Biosciences Show less
- Instituto Politécnico de Bragança
  2017 - 2021
  Bachelor of Engineering - BE Chemical Engineering
  The dissertation was on "Techno-economic analysis of chemical processing plant and designing for heat exchanger network."Also did a Demola project: Formulation of foundation cream. Other modules studied include: - Organic Chemistry - Thermodynamics - Chemical reaction engineering - Fluid dynamics - Microbiology - Mass and heat transfer - Separation processes
Experience
- Instituto Politécnico de Bragança
  Feb 2021 - Jun 2021
  Scientific Initiation Internship (Grade: 17/20 from the panel )
  Supervisor : Professora Filomena Barreiro- Successfully conducted bibliographic research for the preparation of pickering emulsion using polymers.- Formulated the micro-particles of the polymers for the development of Pickering emulsion which has become important in different industries.- Hand-on experience in preparing emulsions.
- FlexSea
  Nov 2023 - Mar 2024
  Bioprocess technology Intern
  - Scaling up of microbial bioprocess from shake flasks to 7L bioreactors and used for the production of sample materials.- Established the Process flow diagram (PFDs), P&ID and Standard Operating Procedures(SOPs). - Responsible for experimental design and screening in a shake flask.- Performing downstream processing of biopolymers using a centrifuge and tangential flow filtration (TFF).- Troubleshooting the equipment/process issues and implementing CAPAs.
- CELLULAR AGRICULTURE LTD
  Apr 2024 - Sept 2024
  Bioprocess Technician
  - Performing aseptic seed train, mammalian cell culture in TC flasks, bioreactors and molecular analysis, protein assays.- Brainstorming novel upstream/downstream bioprocessing strategies to characterize and optimize existing processes.- Generating market reports for equipment/consumables procurement and electronic laboratory notebook maintenance.- Documenting and performing root cause analysis during process anomalies and implementing CAPAs for resolving issues.- Setting up and running test rigs for bioreactors and downstream processing units.- Preparing, following, and/or adapting to standard laboratory operating procedures for continuous improvement and generation of reliable experimental results.- Proactively contributing towards the safety policies and procedures by assessing the work environment and writing documentation Show less
Licenses & Certifications
- Get Started with Python
  Grow with Google on Coursera
  Dec 2023
  View certificate