Tamar Grevelink

Experience

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  HEMA

  Apr 2008 - Jun 2010

  Cashier
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  Van der Valk Hotel Amersfoort A1 and Leusden

  May 2010 - Sept 2011

  Waitress
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  Besseling Vervoert

  Jun 2011 - Sept 2013

  Courier
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  Studiekring Amersfoort

  Nov 2011 - May 2012

  Tutor & homework counsellor
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  UMC Utrecht

  Oct 2012 - Jul 2013

  Research Intern

  In the Laboratory for Translational Immunology (LTI) I studied the influence of D-strand amino acid of the FcαR on the binding capacity for IgA and the role of the FcαR in induction of autophagy.Techniques: PCR, gel electrophorese, site directed mutagenesis, molecular cloning, transfection, retroviral transduction, FACS, ELISA based bindings assays, western blot, fluorescence confocal microscopy, isolation of primary cells, cell culture

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  Eurofins Analytico B.V

  Oct 2013 - Dec 2014

  Laboratory employee

  Tasks:-Preparing samples for analysis-Weight samples and submit to LIMS-Pre-treatment of samples for metal analysis

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  NIZO food research

  Jan 2014 - Jul 2014

  Research Intern

  In the health department of NIZO I've done a 6-month internship where I studied effects of food ingredients, such as probiotics, on intestinal healthTasks:-Optimization and upscaling of current cell-based assays-Developing new assays -Test different food ingredients-Writing SOPsTechniques: Cell culture, FACS, ELISA, TEER (transepithelial electrical resistance) assays and agar plating

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  UniQure

  Jan 2015 - Nov 2024

  March 2017The Vector Development department had merged with the Process Development departmentAdditional responsibilities:- Communication of timelines, process updates and schedules with requestors, team and Assay Development (for sample analysis)-Review of documentation/Batch Production Records -Writing production plans and reports (in global QA system) for a In Vitro Diagnostic (IVD) vector batch -Participate in CMC team for IVD project-Tech transfer to Lexington-Writing of QA documentation for implementation of electronic laboratory notebook (ELN) system2015-March 2017 In the vector development department I am involved in the analysis and generation of AAV based vectors for gene therapy.Tasks:-Analysis, production and purification(USP/DSP) of AAV batches -Support BPU (Baculovirus Production Unit)-Optimization of current procedures and development of new assays -Planning of productions for the team and responsible for availability of start materials for production-Arrange shipments of materials to CROs/US site -Writing reports, worksheets and standard operating procedures (SOPs)-Lab responsible and emergency response officerTechniques: (Q)PCR, TCID50 assays, infectivity/potency assays, SDS-PAGE, western blotting, gel electrophorese, sequencing data analysis (NGS), cell culture (flasks, wavebags, cell factories),affinity chromatography/Ion-exchange chromotography (Äkta), ultrafiltration/diafiltration, HPLC, DLS Show less

  • Project Manager

    Oct 2023 - Nov 2024

  • Global Program Manager

    Jul 2021 - Oct 2023

  • Program Management Coordinator

    Jul 2020 - Jul 2021

  • Coordinator Vector Core/Senior Technician Process Development

    Jan 2019 - Jul 2020

  • Technician Vector Development

    Jan 2015 - Jan 2019
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  Sanofi

  Feb 2025 - now

  Regulatory Affairs Manager