Titto Mathew, PhD

Titto mathew, phd

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location of Titto Mathew, PhDSwansea, Wales, United Kingdom
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  • Timeline

    Jun 2009 - Nov 2009

    Clinical Research Associate

    ALTREE Health care & Research centre,Kochi
    Jan 2010 - Dec 2011

    Jr.Researcher

    Ministry of Defence of India
    Jan 2012 - Jun 2016

    Senior Researcher

    Defence Research and Development Organisation
    Aug 2015 - Jul 2016

    Assistant Professor

    St Berchmans College
    Oct 2016 - Jul 2018

    Research Scientist

    Goverment Medical College Calicut
    Sept 2018 - Mar 2019

    Chief Scientific Officer

    Perumalil Scans and Diagnostics
    Apr 2019 - Feb 2023

    Microbiologist cum Infection Control Specialist

    Satyanand Hospital Pvt ltd
    Current Company
    Jan 2023 - now

    Clinical Microbiology

    Swansea University
  • About me

    Microbiologist cum Infection Control Specialist

  • Education

    • Defence research and development organisation

      2010 - 2016
      Doctor of philosophy (ph.d.) life science

      Thesis - Effect of Hypoxia on alveolar epithelium: Modulation by Curcumin’’Technical experience - PCR, western and far-western analysis, ELISA, Cell culture, EMSA, Oxidation assays, plasma membrane isolation, Alveolar fluid clearance, in-vitro Blood Brian Barrier and Alveolar Epithelial barrier formulation, HPLC, Blue native gel, IFA, RT-PCR, IHC, ICC.Other professional experiences - Scientific styled writing exhibited through writing proposals, grants and manuscript… Show more Thesis - Effect of Hypoxia on alveolar epithelium: Modulation by Curcumin’’Technical experience - PCR, western and far-western analysis, ELISA, Cell culture, EMSA, Oxidation assays, plasma membrane isolation, Alveolar fluid clearance, in-vitro Blood Brian Barrier and Alveolar Epithelial barrier formulation, HPLC, Blue native gel, IFA, RT-PCR, IHC, ICC.Other professional experiences - Scientific styled writing exhibited through writing proposals, grants and manuscript preparation. Show less

    • Alphonsa school

      1998 - 2001
      High school mathematics and computer science, biology
    • Amrita school of biotechnology

      2004 - 2007
      Bachelor’s degree biotechnology first class with distinction

      Dissertation: “Primary Screening for novel inhibitor against V. harveyi from A. indica.”

    • Loyola college

      2007 - 2009
      Master’s degree biotechnology first class

      Dissertation: “Some Immunological studies of GroEL (HSP60) and DnaK (HSP70) of S. Typhi in mice: in vitro and in vivo”Technical experience - protein expression and affinity purification, yeast-two hybrid analysis, Cloning, PCR, Mammalian cell culture (Spleenocytes and mouse embryonic cells), FISH, ELISA.

  • Experience

    • Altree health care & research centre,kochi

      Jun 2009 - Nov 2009
      Clinical research associate

      • Consulted with the Institutional Review Board to present study budgets, objectives and key milestones for study approval• Effectively managed a variety of study aspects including budget oversight, patient screening, recruitment and patient/family counselling and education• Organized and maintained study documents including consent and assent forms, registries and patient charts

    • Ministry of defence of india

      Jan 2010 - Dec 2011
      Jr.researcher

      • Lung physiologist investigating defective ion channel activity associated with the High altitude pulmonary oedema. Specific focus on the interaction between NFkB and ENaC, and rectification of associated channel defects. Showed ability to interact well with clients vendors and other lab partners .• Neurobiologist interested in investigation the Blood brain barrier (BBB) breach under oxidative stress. • Showed novel mechanism of BBB rupture under hypoxia and efficacy of Curcumin in preventing Tight junction impairment under high altitude hypoxia. Show less

    • Defence research and development organisation

      Jan 2012 - Jun 2016
      Senior researcher

      Lung physiologist investigating defective ion channel activity associated with the High altitude pulmonary oedema. Specific focus on the interaction between NFkB and ENaC, and rectification of associated channel defects. • Developed project to produce novel data within the pulmonary infection field generating an invitation to present an oral communication at the Society For Free Radical Biology And Medicine (SFRBM) 21st Annual Meeting• Showed ability to interact well with clients resulting in collaboration with two major pharmaceutical companies with sustained frequent client interaction. • Expert in cell culture techniques, with over 4+ years experience in culture of multiple cell lines and primary epithelial cell, including culture at air-liquid interface. Competent in transient cell transfection using multiple transfection systems, allowing study of ion channel physiology. • Planned, developed and executed experimental protocols using multiple techniques including western blot, qPCR, immunocytochemistry, confocal microscopy, super resolution microscopy, airway surface liquid height measurements, surface biotinylation and, absorbance/fluorescence/imaging based high throughput plate reader assays. Show less

    • St berchmans college

      Aug 2015 - Jul 2016
      Assistant professor

      • Adept public speaker as evidenced by delivering 3hrs daily lecture. Mentored 8 undergraduate and 5 Masters students for the establishment of projects and also in teaching of different molecular biology techniques.Formation of curriculum, instruction plan and lab manuals.• Subject handled o Undergraduate students: Animal biotechnology and Bio-nanotechnology, Cell biology, Microbiology, Biostatics and Industrial biotechnology. o Masters: rDNA technology, Biostatics, Biophysics, Environmental Science. • Labs handled: o UG: Microbiology, Microbial technology and Enzymology, Animal biotechnology. o Masters: Microbiology, Molecular Biology, rDNA technology. Show less

    • Goverment medical college calicut

      Oct 2016 - Jul 2018
      Research scientist

      Establishment of state level referral lab for Molecular diagnostic and virus research.Involved in quantification of onolocgy markers (125, CA15-3, CA19-9) HLA typing, Beta & Alpha -thalassemia, Cystic Fibrosis diagnosis, Quantification and diagnosis of various viral diseases ( HBV,HCV, HPV, CMV, RSV, H1N1,H1N5, Zika etc) Genotyping, Genetic Sequencing, FISH & Karyotyping.Expertise on ABI Real Time- PCR 7500fast, BioRad CFx 96 IVD, Ion S5 and Ion S5 XL Systems. BD FACSCALIBUR flowcyometry, HPLC, QIA Symphony, Bioneer automated extraction system.• Project Management: Currently leading Govt. of India Project worth $100,000 with multi-disciplinary professionals to develop a platform for viral research and molecular diagnosis. Ensured deliverables such as progress reports, scientific abstracts and manuscripts were prepared and submitted in timely manner.• Outlined, coordinated and managed studies with academic collaborators to maintain efficient and effective experimentation and data analysis. Excellent mentoring skills demonstrated by supervising 2 graduate students, 2 technicians and numerous interns.• Lab Manager: Contributed to the productivity and smooth running of the lab by coordinating lab meetings, ordering lab supplies, creating and maintaining standard operating protocols and NABL protocols for all laboratory activities, enforcing QA, QC, regulatory and safety regulations and training to Clinicians, Masters students etc. Preformed internal audits on central fund available in the laboratory.Procurement In-charge – To order laboratory instruments, reagents and consumables and ensure that stocks are well organized and controlled Stock Management – Maintaining and updating inventory Show less

    • Perumalil scans and diagnostics

      Sept 2018 - Mar 2019
      Chief scientific officer

      Lead scientist at a new startup managing setup and operation of new laboratory facilities, siteplanning, equipment setup and maintenance. Write and maintain GLP and SOP, ensure OSHA and other safety compliance.Drive the strategic direction of the research services that include the entire spectrum of NGS applications (e.g. whole exome, whole genome, whole transcriptome, metagenome/microbiome, ChIP, targeted amplicon, smallRNA, HiC sequencing) and analyses. Instrumental in executing new initiative of establishing bioinformatics services, including automated pipelines and publication support. Show less

    • Satyanand hospital pvt ltd

      Apr 2019 - Feb 2023
      Microbiologist cum infection control specialist

      • Maintenance and Reporting of Microbial infections in patients – Validation of antibiotic susceptibility and microbial identification using Vitek2 system. • Ensuring hospital infection control surveillance, biomedical waste management and smooth operation of the laboratory facilities. Supervision and training of health care personals (team of 100 members) at different levels. Reporting of molecular diagnostic examination of viral and oncological samples.• Initiated Covid-19 testing, reporting and orchestrated Covid-19 Infection control Show less

    • Swansea university

      Jan 2023 - now
      Clinical microbiology
  • Licenses & Certifications

    • American clinical pathology association

    • Ascp board of certification

      American society for clinical pathology (ascp)
    • Ielts band 7.5 ( speaking 7.5, reading 8.5, writing 6.5, listening 8)

      British council
      Jan 2022
    • Molecular diagnostics

      World education services
      Nov 2020
  • Honors & Awards

    • Awarded to Titto Mathew, PhD
      Surg. Malhotra award for best publication Defence Research and Development Organisation (DRDO) , Govt. of India Sep 2013
    • Awarded to Titto Mathew, PhD
      Travel award Department of Biotechnology 2013
    • Awarded to Titto Mathew, PhD
      Senior Research Fellowship Defence Research and Development Organisation (DRDO) , Govt. of India Jan 2011
    • Awarded to Titto Mathew, PhD
      Jr. Research fellowship Defence Research and Development Organisation (DRDO) , Govt. of India Dec 2009
    • Awarded to Titto Mathew, PhD
      Rank holder Amrita University May 2007
  • Volunteer Experience

    • Conference orginisation

      Issued by Defence Research and Development Organisation
      Defence Research and Development OrganisationAssociated with Titto Mathew, PhD