Daniele De Simone

Experience

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  University of Birmingham

  Sept 2019 - Aug 2020

  Research Technician

  Project: Investigate the role of the specific lipid in the activation of invariant Natural Killer T cells and identify the role of the protein CD1d on Acute Myeloid Leukaemia cell lines and blasts.DNA\RNA extraction (culture cell) and quantificationPCR, RTWestern blot, SDS-PAGE, Protein extraction (culture cell)ELISA test: Sandwich ELISA test (cell culture media)Flow cytometry ("Cytoflex" by "Beckman Coulter")Lymphoprep separation of cells from healthy donors and patient bloodMagnetic-activated cell sortingGeneration, differentiation and expansion of iNKT cells from healthy donorsEukaryote cells culturing and transfectionPlasmid DNA isolation (Mini/Midi prep) Show less

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  Dante Labs

  Sept 2020 - Aug 2021

  Analytical scientist

  Handling and analysis of SARS-CoV-2 samples.Samples transfer in multiwell plates by Janus G3, Beads RNA extraction by CheMagic 360 and MGISTP-960, Real Time PCR, detection of SARS-Cov-2 variants, data analysis.Preparation and analysis of biological samples (human serum, swabs, dried blood spots, etc) for the detection of: anti-coronavirus antibodies (IgG, IgM and IgA) using "EuroLab Workstation ELISA" (Euroimmune), food allergies and intolerances using "EuroBlotOne" and "EuroLine Scan" (Euroimmune) Show less

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  GSK

  Oct 2021 - now

  Associate scientist

  Study of GMMA-based vaccines against infectious diseases affecting low- and middle-income countries. Serological analysis of pre-clinical (mice, rats, rabbits) and clinical samples in order to evaluate the level, quality and functionality of antibodies by:- High-throughput ELISA performed in 96 and 384 format using Hamilton and Zephir workstation- Multiplex assays for measuring proteins target by Luminex workstation (xMAP Technologies)- High-throughtput Luminescence Serum Bactericidal Assay (L-SBA)- Opsonophagocytic killing assay (OPK)Others tasks:- Contributing to the conception, design, and interpretation of scientific data in support of research projects- Prepare and presents scientific data in internal and external meeting- Participate in writing technical protocols and reports in support of various project development stages. Show less