Jayonta Bhattacharjee

Experience

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  Field Fertility Clinic, Dep. of Surgery&Obstetrics, Bangladesh Agricultural Univerisity, Bangladesh

  Dec 2005 - Sept 2007

  Scientific Officer

  Worked for implementing policy and techniques for the improvement of the dairy industry.Organized dairy farmers with cooperatives and provided veterinary services to smallholder farmers. Developed communication skills with different peoples including farmers and policymakers.

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  Università degli Studi di Siena

  Oct 2007 - Oct 2010

  PhD Researcher

  #Planned, designed experiment protocols, laboratory skills, set timelines and completed research work and statistical analysis of 4 projects in 3 years that led to the discovery of role and function of ABCA1 in human placenta. The University of Siena and the European Union funded the projects.#Developed expertise on tissue processing by performing placenta tissue dissection, processing and slide preparation for the immunohistological examination#Established proficiencies on placental explants culture for ex vivo placenta experiments#Developed hands-on experiences on total and membrane protein extraction and RNA extraction from placental tissues#Participated in statistical analysis courses implemented by the doctoral school #The independent nature and originality of the projects led to 9 scientific publications in high profile peer-reviewed journals#Developed scientific writing skills led to write an extensive review on pro-inflammatory cytokines during gestation#Presented in local and international scientific meetings#All the experiments and performances led to the generation of the best Ph.D. Thesis of the academic year by the Ph.D. School of Molecular and Cellular Physiology, Pharmacology and Toxicology#Technical/Assay Development: •Developed new experimental protocol and procedure along with the existing protocols to meet the experimental goals•Performed both cell-based and tissue-based assays includes cDNA library generation, RT-PCR, PCR Primer design, ELISA, EIA, Bradford assays, western blot, immunohistochemistry, immunofluorescence, gel electrophoresis•Trophoblast cell and explant culture and maintenance•Cryogenic tissue preparation for cryosection•Performed MTT assay, alamarBlue assays for cell activity and viability test•Accomplished confocal microscopy for localization and co-localization of proteins in tissues and explants Show less

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  University of Rome "La Sapienza"

  Nov 2010 - Feb 2011

  Research Scholar

  # Took part in collaborative research between 2 universities in Italy for 4 months# Worked on Macrophage migration inhibitory factor (MIF) in placental development and diseases# Developed collaborative and communication skills

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  Mount Sinai Hospital (Toronto), Sinai Health System

  Mar 2011 - Nov 2013

  Post-Doctoral Fellow

  #Designed research protocols after the literature review, executed experiments, statistical analysis led to the discovery of dynamic regulation of 3 important proteins (HIF1, FIH, and Mcl-1) in human placenta and trophoblast cells resulting in 2 scientific publications in peer-reviewed journals #Developed grant writing skills and successfully wrote and won a research grant of CAD 154,000 from CIHR as a scholarship holder #Presented at 7 local and international scientific meetings#Developed manuscript writing skill#Technical/Assay Development: Developed sound laboratory skills, planned, completed and developed expertise on western blot, immunoprecipitation, RNA extraction, PCR, silencing, bacterial culture, transfection, trophoblast cell culture and maintenance, immunofluorences, immunohistochemistry and immunocytochemistry, fluorescence and deconvolution microscopy Show less

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  Bangladesh Agricultural University

  Jan 2012 - May 2018

  Faculty

  # Teaching and Research# Established management and administrative skills working as Executive Director of Community-based Dairy Veterinary Foundation for 4+ years.# Developed project management, report writing and communications skills working as a principal investigator of 4 research projects of CAD 44000.# Worked as a team player, problem solver and successful supervisor as evidenced by the graduation of 7 masters degree graduates. # Experienced in interviewing, collecting and managing scientific data, qualitative and qualitative research as demonstrated by completion of 4 research projects with multiple reports in each, 7 master degree thesis and 3 peer-reviewed publications. # Worked as a good organizer by organizing 4+ training programs for the farmers. Show less

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  University of Ottawa

  Aug 2018 - Jun 2021

  Postdoctoral Researcher and Tech, Prevention in the Early Years research program

  # Working in a multidisciplinary group and developed sound communications skills by sampling from 5 different hospitals and birthing unit of Ottawa city.# Achieved independent project management and research establishment ability working in multi-tiered approached research projects. # Exhibited problem-solving capacity in establishing 3+ experimental protocols for protein and DNA/RNA analysis in instituting new wet lab facility. # Tools: Till now using: Human placenta tissue model. Collecting placenta from study participants using the recommended guidelines of GCP. SPS-PAGE, Western blots, IHC, DNA/RNA extraction, PCR, PCR Primer Design tools. Using ChemicDoc XRS system for visualization of protein and DNA/RNA blots and gels. Troubleshooting and validating antibody used for different experiments.# Using my previous skills in project management and effectively maintaining my reseach project. Show less

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  Bangladesh Agricultural University

  Jun 2021 - now

  Faculty

  # Teaching and Research# Established management and administrative skills working as Executive Director of Community-based Dairy Veterinary Foundation for 4+ years.# Developed project management, report writing and communications skills working as a principal investigator of 4 research projects of CAD 44000.# Worked as a team player, problem solver and successful supervisor as evidenced by the graduation of 7 masters degree graduates. # Experienced in interviewing, collecting and managing scientific data, qualitative and qualitative research as demonstrated by completion of 4 research projects with multiple reports in each, 7 master degree thesis and 3 peer-reviewed publications. # Worked as a good organizer by organizing 4+ training programs for the farmers. Show less