ISHAN SHARMA

Experience

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  Jaypee University of Information Technology, Waknaghat

  Jun 2014 - Jul 2014

  Trainee

  Molecular Biology TechniquesPlasmid isolation, Restriction analysis, Cloning and PCR amplification of a given gene, Molecular marker techniques

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  Institute of genomics and integrative biology (CSIR)

  Jul 2015 - Dec 2015

  Trainee

  Understanding of skin cell Homeostasis using cell culture models Under this project, one of the goals was to understand physiological functions of certain keratinocyte specific genes in vivo using transgenic animal model. Towards this, we aim to generate transgenic mice carrying KGF or Fibroblast Growth Factor-10, commonly known as KGF (Keratinocyte Growth Factor) secreted by Dermal fibroblast by Developing the transgenic mice using Spermatogonial Stem cells. Also, Study of Organelle dynamics in skin cells for Mitochondrial damage rescue from UV Stress and Development of tools to study Autophagosomes found in skin cells by Expression and Purification of Autophagy specific Atg17 protein. Show less

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  Panacea Biotec

  Mar 2017 - May 2018

  Scientific Officer

  Job Responsibilities 1. Involved in Bacterial Vaccine Production In Upstream (Fermentation) & Downstream Processing. 2. Validation and Revalidation of Fermenters, TFF's, Process Vessels, HVAC, Autoclave3. Preparation & review of BMR's4. Media, Buffer preparation and dispensing activities 5. Performing CIP, SIP, PHT and periodic cleaning of all manufacturing systems which include Fermenters, TFF, Media Vessels, Preparation vessels, Buffer vessels etc. 6. Performing Cleaning validation and Product changeover cycles along Show less

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  Intas Pharmaceuticals

  Jun 2018 - Jul 2019

  Executive

  Job Responsibilities 1. Microbial Fermentation (Upstream Processing)2. Validation and Re-validation of Process Equipment's3. SIP validation of Fermenter, Media Fill activities4. Media and Buffer Preparation. 5. Performing CIP, SIP, PHT and periodic cleaning of all manufacturing systems which include Fermenters, Media Vessels etc. 6. Part of QMS team, Handling Incident, Deviations and CAPA.

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  DD Enterprises

  Aug 2019 - May 2022

  Senior BioProcess Engineer

  Job responsibilities 1. Post project handover from BD, working closely with project team to plan project kick off meet with client (off site/ on site/ online) to discuss project technicality & feasibility.2. URS, P&ID Review and providing necessary inputs from an holistic perspective. Review & compliance on clients inputs received post technical discussions.3. P&ID Standardization, P&ID preparation , Component selection assistance based on the process requirement.4. 3D model review for process, holdup, deadleg and cold spots in the skid.Drawing standardization, SOP preparation for day to day activities of review & approval5. Line sizing, URS, Vendor Development, Utility calculations, Pump selection, working closely with various department to ensure smooth process flow.6. Assistance to Technical Sales team for system designing during bidding stage.7. Input/ assistance to process automation team for designing recipe.8. Assistance to pre FAT and FAT team to cross verify the built skid.9. Assistance to site team for SAT of the system.10. Commissioning Qualification & validation of Various Biopharma & pharma systems ( Fermenter, Bioreactor, TFF, Process vessels, Filtration skids, MDI Systems etc.) and troubleshooting. 11. Preforming all sorts of sequences in the automation Package under OQ i.e. CIP, SIP, PHT, ISIP, TLSIP, TLCIP, Fermentation, Batch process etc. 12. Well versed with all activities involved during IQ,OQ & PQ of Pharma and Biopharma systems. Show less

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  NNE

  Jun 2022 - Feb 2023

  Process Engineer

  Responsible for following activities1. Design and analysis of Bioprocess, which involves upstream processing, downstream purifications and processing equipment like fermenters, bioreactors, clean utility, autoclaves, TFF etc., which helps to design CIP / SIP system.2. Basic P&ID preparation, understanding the key elements in P&ID, P&ID flow path, extended P&ID review in 2D & 3D format and equipment layout drawings review.3. Design and analysis of process piping requirements, pipe sizing, clean and black piping requirements, pipe flow & pressure drop calculation, etc.,4. Utility calculations, Material balance, Energy balance calculations.5. CIP / SIP system and other process equipment design review against International regulatory Standards, such as ASME, BPE, ASTM, ISPE, PDA, basic safety codes6.Design and analysis of mountings like sanitary valves, PRV, mixers, instruments, pump, vent filters, etc.,7. Commissioning and Qualification support. Show less

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  Biocon Biologics

  Mar 2023 - now

  Deputy Manager Projects