Krista Hu

Experience

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  Lexplorations Chamber Orchestra

  Jul 2013 - Aug 2013

  Teaching Aide

  Supervision of elementary and middle school students during recreational time. Individual coaching of small group ensembles and teaching assistance during large ensemble rehearsal, as well as individual leading of sectionals from the large ensemble.

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  Brandeis University

  Feb 2014 - May 2017

  • Making diets and feeding lab animals• Separating lab animals (young from parents and splitting cages)• Measuring blood glucose levels in lab animals• Preparation of tubes for sample collection for necroscopies• Taking tissue samples and recording data during necroscopies• Separation of plasma samples from lab animals• Working independently on data collection and analysis for assigned experiments• Training and supervising new volunteers in lab procedures• Cleaning the facilities Show less

  • Organic Chemistry Lab Teaching Assistant

    Aug 2016 - May 2017

  • Undergraduate Research Assistant

    Feb 2014 - May 2017
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  Wilson Hsin Dental Associates: Hsin Wilson DDS

  May 2014 - Aug 2014

  Summer Intern

  Sanitization and preparation of equipment, development of x-ray film, shadowing and assisting general dentist during procedures such as cleanings, extractions and fillings.

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  The Rivers School Conservatory

  Jul 2014 - Aug 2014

  Summer Camp Counselor

  Supervision and instruction for six to nine-year olds during individual instrumental practice time, direct assistance in musical instruction of kids throughout each day, collaborating with three other peers to maintain a safe, educational environment for a group of 48 kids during two successive two-week sessions of day camp.

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  Massachusetts General Hospital

  May 2016 - Aug 2016

  Research Intern

  • Independent development of new FISH technique - use of fiber FISH and IF-FISH• Attending and presenting work at weekly lab meetings• Tissue culture - thawing, growing, freezing down and splitting tumor cells• Slide visualization using microscope and CytoVision software• Assisting technicians in any and all duties, including cDNA synthesis, Repli-G, PCR, making buffers, diluting reagents, inventory

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  Massachusetts General Hospital

  Jun 2017 - Jun 2019

  Research Technician I

  • Next generation sequencing using AMP technology from ArcherDx • Library preparation of DNA and RNA for Solid Fusion Assay and SNaPshot Assay • Using Kapa Biosystems kits for qPCR with the Applied Biosystems 7500 Real Time PCR System • Using the Illumina MiSeq for in-house NGS • Analysis of sequencing data by running an in-house pipeline• Managerial duties including ordering materials, maintaining lab equipment and enforcing proper lab protocol• Presenting data at weekly lab meetings Show less

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  Columbia University Irving Medical Center

  Aug 2019 - now

  Doctoral Student

  • Responsibilities: Propose, design, optimize, and execute experiments, analyze results. Orally communicate hypotheses and findings in lab meetings and research seminars. Mentor and train two undergraduate students. Write, review, and edit predoctoral fellowship applications and research articles. Lead weekly 1 on 1 meetings with PI.• Methods: mouse neonate dissection, adult mouse dissection of adipose depots and liver, isolation and culture of stromal vascular fraction cells from adipose, in vitro and in vivo lipolysis assays, glucose tolerance tests, FPLC, lipid assays, animal husbandry and mouse colony maintenance, sectioning of paraffin-embedded tissue, TUNEL staining, cloning of doxycycline-inducible overexpression cell lines, adipocyte culture and differentiation, siRNA-mediated knockdown, immunoprecipitation, TaqMan-based qPCR, ELISA• Wrote, reviewed and edited 5 fellowship applications to secure 2 years of funding, awarded 2 fellowships Show less