Gajraj Rathore

Experience

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  L V PRASAD EYE INSTITUTE

  Jan 2017 - Jul 2017

  Summer Intern

  Worked under the guidance of Dr V.S Sangwan, on the topic of Simple Limbal Epithelial Transplantation, with the aim of developing a scalable stem cell based treatment for damage to outer ocular surface.

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  PANACEA, Pharmacy Association

  Apr 2017 - Jan 2018

  President
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  Université de Sherbrooke

  Jan 2018 - Aug 2018

  Summer Internship under Professor Brendan Bell

  The aim of the project was to determine the role of caspases in inducing apoptosis through the activation of TAF6δ, an inducible pro-apoptotic subunit of the general RNA polymerase II transcription factor, TFIID. I tested multiple cell lines(HeLa, Jurkat, MDA-MB-231, HL-60) for their response to inducers of apoptosis upon caspase cleavage of model substrates. During the course of this project, I mastered mammalian cell culture, protein extraction and quantification and Western Blotting, all of which were extensively used throughout my internship. Weniger anzeigen

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  A*STAR - Agency for Science, Technology and Research

  Feb 2019 - Jul 2019

  Research Assistantship under Dr Lakshmanan Manikandan

  As a Research Assistant at the Institute of Molecular and Cell Biology, my role was to perform in-vivo studies in mice (BALB/c) with the aim of elucidating the molecular mechanisms involved in immune checkpoint inhibition, a popular approach in cancer immunotherapy. I was directly involved in experimental design which involved injection of tumor cell lines (Syngeneic cell lines) in mice and monitoring their growth over time. Immune profiling was performed on the tumor extracted after 14 days, through CyTOF mass cytometer. We further employed the use of PD-1 inhibitor antibody to view its effects on tumor growth. The results were promising as the tumor volume showed significant reduction after drug treatment. During the project, I gained hands on experience with state-of-the-art techniques such as LC-MS/MS, which was used for pharmacokinetic studies and it was my first experience of working in pre-clinical environment which further motivated me to work towards translational research. Weniger anzeigen

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  Université de Genève

  Sept 2020 - Feb 2021

  Master Thesis Student under Professor Yogeshvar Kalia

  During my master thesis at the University of Geneva, I worked on the non-invasive topical delivery of Infliximab for the treatment of psoriasis. We developed ELISA for antibody quantification which was validated for its accuracy and precision. It gave me insight into ICH validation guidelines for analytical method development. For antibody-drug delivery, our focus was on using iontophoresis for reaching deposition level above the therapeutically relevant value of 100xEC50. We employed the cutaneous bio-distribution method for precise drug profiling in the skin and were able to develop an iontophoretic patch prototype. The process of device development was very intriguing and gave me the opportunity to work in an inter-disciplinary environment. The prototype will be used for carrying out topical delivery in vivo, planned for later 2021. Weniger anzeigen

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  Ender diagnostics ag

  Jan 2021 - Feb 2023

  Head of Laboratory

  Being a part of a dynamic start-up, I enjoy challenges with regard to operational processes in the lab. The responsibilities on a daily basis are summarized as follows: • Monitor lab workflow, ensure high-quality and efficient services to the clients• Ensure compliance quality management system (ISO 13485) and maintain SOPs• Resolve conflicts within the team and maintain fluent communication• Ensure training and evaluation of employees, oversee continuous process improvementIn this managerial position, I oversee various projects including supply chain management, establishing Quality Management System, and setting up the IT infrastructure for the lab. It has enhanced my critical thinking abilities and cultivated a reputation as a major contributor through continuous improvement and time management. Weniger anzeigen

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  Ichnos Glenmark Innovation

  Apr 2021 - Sept 2021

  Intern, Analytical Method Development

  I was a research intern in the Drug Product Development team wherein I routinely worked with analytical techniques such as Protein A-HPLC, Cation Exchange Chromatography,Capillary Electrophoresis, SEC-HPLC, Endosafe PTS for purification and characterization of antibody samples. I also worked on method development for analysing sialic acid in glycoproteins using UPLC-FLR (Ultra Performance Liquid Chromatography-Fluorescence Detector). Apart from gaining hands-on experience with analytical techniques, this experience gave me an insight into the pharmaceutical industry. I actively participated in maintaining large datasets of sample inquiries, writing SOP, building fluent communication across the team. I firmly believe that these skills have helped me to acclimatize to new working environments and contribute towards successful completion of complex tasks. Weniger anzeigen

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  Biognosys

  Jan 2023 - now

  • Quality Manager Operations

    Aug 2023 - now

  • Laboratory Manager

    Jan 2023 - Aug 2023