Roberto De Gregorio

Experience

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  Consiglio Nazionale delle Ricerche

  Nov 2010 - Apr 2016

  Project: Characterized candidate miRNAs tissue expression in mouse brain and assessed the impact of their overexpression in mES and MEF on the in vitro differentiation efficiency to dopaminergic neuron.• Trained 3 graduate/undergraduate students and 2 apprentices in cell culture, molecular biology, histology and biochemistry techniques. Advised students on proper thesis writing and preparation for oral presentation.• Prepared and taught lessons on the “Development of the Nervous System” to Biology and Biological Science undergraduate and graduate students as part of a Neurobiology course.• Supervised cell culture room shared by 4 independent labs (about 20-25 users). Show less • Identified miRNAs involved in dopaminergic neuron differentiation through high-throughput miRNA/mRNA microarray screening approach, and paired bioinformatic analyses, on in vitro stem cell differentiation models.• Validated candidate miRNAs and characterized their function and targets, through lentivirus-mediated miRNAs overexpression on differentiating cell models, including iPSC and MEF, and subsequent phenotypic screening and characterization.• Acquired stem cell culture skills during the visiting researcher period and transferred the know-how to the main laboratory by setting up equipment and material and training colleagues.• Independently developed and started the main project but established multiple collaborations to fulfill the necessity of specific expertise. Involved in two side projects by providing molecular biology and stem cell expertise. Show less

  • Research Collaborator, Research Trainer/Supervisor, Molecular Biology, Stem Cell Neurobiology

    Apr 2014 - Apr 2016

  • Researcher Ph.D. Student, Molecular Biology, Stem Cell Neurobiology

    Nov 2010 - Apr 2014
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  Imperial College London

  Jul 2011 - Oct 2012

  Visiting PhD Student Researcher, Stem Cell Neurobiology
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  Albert Einstein College of Medicine

  Sept 2016 - Jul 2022

  • Pioneered studies to uncover the function of SERT transient expression in unique non-serotonergic neurons during brain perinatal development, using SERT conditional KO mice, a potential mouse model for autism spectrum disorder and/or other neurodevelopmental disorders.• Introduced and implemented new techniques to current laboratory, including single-cell RNA-Seq, RNAscope in situ hybridization, AAV stereotaxic injections, neuronal circuit tracing and dendritic morphometric analyses. • Worked independently on multiple projects. Wrote two manuscripts and contributed to grant writing.• Established external collaborations with experts on electrophysiology, bioinformatics and proteomics to pursue the project and explore novel project directions. Show less

  • Faculty Research Associate, In vivo Developmental Neuroscience

    Aug 2021 - Jul 2022

  • Postdoctoral Research Scientist, In vivo Developmental Neuroscience

    Sept 2016 - Aug 2021
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  Weill Cornell Medicine

  Jul 2022 - now

  Senior Research Scientist

  • Leading the establishment, testing, and implementation of new spatial profiling platforms. This includes, but is not limited to, liaising with the companies, organizing machine installations, managing reagent and equipment orders, overseeing inventory, developing protocols, estimating service price, managing projects' timelines, executing initial trial experiments and subsequent training of junior personnel, connecting with application scientists for updates and troubleshooting, and pre-release data quality control. • Providing scientific support to Weill Cornell Medicine MDs and researchers interested in utilizing spatial profiling technologies (10x Genomics Visium, Nanostring CosMx). This involves email correspondence, remote meetings, and preparing instructional materials to describe the technology and guide the sample preparation. • Actively participating in both the organizational aspects and hands-on execution of the Spatial Atlas of Human Anatomy (SAHA) program, a collaborative effort involving the EIPM, the WCM Department of Pathology, the WorldQuant Initiative, and Nanostring. Show less