Mariana Carvalho

Experience

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  Projeta Jr

  May 2011 - May 2013

  Vice President

  Junior Enterprise - Engineering Consulting Company

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  Petrobras

  Aug 2011 - Feb 2012

  Estagiária de Projeto de Processos

  Estudo e elaboração de projetos básicos e executivos, junto com o engenheiro de processamento do setor, da produção dos diversos campos. Participação da elaboração dos Projetos de Combate a Incêndio e Pânico das estações. Elaboração de fluxogramas de engenharia, fluxogramas de processos, isométricos, planta de tubulações. Visitas a campos e estações de produção.

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  Lades - PEQ/COPPE

  Feb 2014 - Feb 2016

  Research Assistant, Master Student

  • Conducted research on modeling and simulation of an industrial ammonia synthesis reactor using EMSO (Environment for Modeling, Simulation, and Optimization) simulator.• Developed a robust mathematical model for a large-scale ammonia reactor (Topsoe), modeling the radial flow through the catalyst beds and the heaters exchanges present inside the converter, considering mass, heat, and momentum balances.• Provided client solutions through the development of performance analyses, assessing the impact of specific process variables to determine optimal operational conditions.• Presented a novel advanced model, using GAMS software, to optimize oil production in wells with insufficient reservoir pressure employing gas lift technology. Show less

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  Petrobras

  Mar 2014 - Feb 2016

  Graduate Researcher

  • Conducted research on modeling and simulation of an industrial ammonia synthesis reactor. • Developed a robust mathematical model for a large-scale ammonia reactor (Topsoe), modeling the radial flow through the catalyst beds and the heaters exchanges present inside the converter, considering mass, heat, and momentum balances.• Development of performance analyses, assessing the impact of specific process variables to determine optimal operational conditions.

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  Casa Noronha Produtos de Limpeza

  Sept 2016 - Mar 2017

  Chemical Process Engineer

  Casa Noronha is a manufacturer of cleaning and sanitizing products, situated in Colatina, ES, Brazil.• Led a team of 12+ workers, overseeing end-to-end production operations. Skillfully synchronized production with demand, adeptly addressed client requisitions, and engaged in strategic negotiations with suppliers for optimal raw material procurement.• Executed GMP and quality control assessments on incoming raw materials, packaging, and final products. Conducted comprehensive laboratory analyses, including pH and viscosity measurements, ensuring adherence to stringent quality standards• Applied and oversaw the regulatory registration of manufactured products, ensuring compliance with the Brazilian Health Regulatory Agency (ANVISA) guidelines. Successfully secured licenses within federal policies for safely handling toxic and hazardous raw materials. • Designed a waste management system to effectively treat both solid and liquid industrial waste. Show less

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  MilliporeSigma

  May 2017 - Sept 2021

  Doctoral Researcher

  • Applied statistical analysis and design of experiments for the development of new media formulation for CHO cell culture.• Applied dynamic metabolic flux, kinetic dynamics, data-driven, and hybrid modeling to reproduce fed-batch, intensified fed-batch, and perfusion for CHO cell culture bioreactor systems. • Analysed media formulation influence on CHO cell culture performance and titer production.• Developed of new media formulation for maximization of mAb production in CHO cell culture through multivariate statistical analyses. The proposed new media formulation doubled the total titer production. Show less

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  University of Waterloo

  Sept 2017 - Sept 2021

  • Applied a hybrid (mechanist and empirical) modeling approach for modeling of fed-batch CHO cell culture bioreactor. Incorporated media formulation influence on kinetic dynamics using MatLab script language.• Developed a dynamic metabolic flux model for CHO cells using an LP model able to predict biomass production for batch and perfusion continuous systems, using Matlab and IBM ILOG CPLEX toolbox.• Developed of new media formulation for CHO cell culture through experimental data analysis using design of experiments, multivariate statistical analyses (PCA and PLS), and regularization regression methods. Proposed new media formulation that doubled the total titer production.• Performed cell culture experiments and High-Performance Liquid Chromatography (HPLC) analysis. Show less • Teaching Assistant for the courses: Strategies for Process Improvement and Product Development, Statistics in Engineering, Numerical Methods for Process Analysis and Design, Applications of Heat and Mass Transfer, Chemistry for Engineers, and Chemical Engineering Concepts 2.• 2+ years of experience as a Teaching Assistant, delivering in-class lectures and tutorials for classes of 70+ students. Demonstrated leading and interpersonal skills while leading and discussing course topics with students and helping them solve challenging problems.• Taught classes and tutorials, and discussed course topics and problems with students. Marked assignments, quizzes, mid-term and final exams. Show less

  • Ph.D. Student, Doctoral Researcher

    May 2017 - Sept 2021

  • Teacher Assistant

    Sept 2017 - Dec 2020
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  Meatleo

  Sept 2021 - Nov 2021

  Consultant - Chemical Engineer, Scientist

  Meatleo is a cell-cultured meat biotechnology company.• Engaged in establishing connections with potential suppliers, as well as collaborating closely with industrial and academic research teams around the world testing their products and cultivation processes.• Engaged in the research, analysis, and development of serum-free media for the cell-cultured meat production process, employing methodologies such as design of experiments and data analysis.• Analyzed the viability of producing cell-cultured meat process and feasibility of manufacturing in large scale. Show less

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  Merck

  Dec 2021 - now

  Senior Specialist , Engineering

  • Employed data analysis, mechanistic and data-driven mathematical models to support upstream process development, upscaling, technology transfer, process deviation analysis, and global technical operations of biologic products at small- and large-scale manufacturing.• Collaborated closely with the experimental and statistical teams to devise a new operational condition for a large-scale bioreactor, mitigating the risk of batch production rejection. • Applied mathematical modeling to assist the supply chain team in making strategic decisions regarding the selection of manufacturing operations for a large-scale implementation. • Formulated a first principal model to propose novel optimal conditions for an upstream bioreactor. Show less