Prakash Shee

Experience

• 

  Indian Institute of Technology, Bombay

  Jul 2010 - May 2012

  Masters Student Researcher

  • Synthesized C3 and C4 symmetrical polycyclic amino acids via Palladium catalyzed cross-coupling reactions (Heck, Suzuki coupling).• Presented the research work in two seminars and wrote a dissertation to defend my Master’s Degree.

• 

  Michigan State University

  Sept 2012 - Jul 2020

   7 years of experience in routine maintenance, instrumental troubleshooting and technical repairing, and training of group instruments: LC-ESI-MS/MS, MRM (Waters G2-XS-QTOF, Xevo TQ-S), GC-MS (Agilent, achiral, chiral), HPLC (Agilent, normal, reversed phase, chiral), Ultracentrifuge (Beckman-Coulter), and UV-VIS (Beckman-Coulter) that were shared by a team of co-workers.  Safety coordinator and person-in-charge for a BSL-1 facility laboratory for 6 years: maintained material safety data sheet (MSDS), chemical inventory, ordered lab supplies, chemicals, and replacement parts for instruments, experienced in safe handling of toxic, corrosive, and flammable chemicals and gases. Trained colleagues with safe handling of bacterial cell culture techniques. Skilled in using techniques like High vacuum Schlenk line, Parr Hydrogenator, NMR (1D and 2D, 500 MHz), Ozonator, FT-IR, Polarimeter, and Flash Column Chromatography. Excellent verbal and written communication skills and expertise in analysis of large set of data, and explanation of results in the form of scientific documentation. Rich experience working in Molecular Biology: cloning, expression and purification of proteins, affinity column chromatography, Michaelis-Menten enzyme kinetics, SDS-PAGE electrophoresis, NanoDrop Spectrophotometer, and protein modelling using software packages like PyMOL, Chimera, Swiss Model, and ligand docking using AutoDock Vina and AutoDock Tools. Initiated a collaboration where I developed an assay for the detection and quantification (GC/MS) of a group of methoxypyrazines, the major component considered to cause Potato Taste Defect in Rwandan coffee beans (Joseph Bigirimana, Gut Lab, Department of Entomology, MSU and Jean Bosco Shingiro, Bourquin Lab, Department of Food Science and Human Nutrition, MSU).  Collaborated with Conagen Inc. (Bedford, MA) on Elucidating the Biosynthetic Pathway of Pyrazines in Bacteria. Show less  Developed a biocatalytic trans-amination route to produce arylserine analogs from a library of substituted cinnamate epoxides using Taxus canadensis Phenylalanine Aminomutase (TcPAM). Synthesized, characterized, and purified a library of substituted cinnamate epoxides (achiral and asymmetric epoxidation reactions) and ring-substituted arylserines (using aldol condensation). Developed a novel method to establish the stereochemistry (threo vs erythro) of arylserine analogs using NMR spectroscopy. Also developed the first conclusive method to separate and characterize every diastereomers of arylserine analogs using GC-MS through chiral auxiliary derivatization.  Deciphered the mechanism of α/β-amination from a surrogate amine donor to an epoxide backbone using ping-pong enzyme kinetics combined with protein-ligand docking. Investigated the mechanism and factors resulting in Potato Taste Defect in Rwandan Coffee Beans in a collaborative project where I contributed in developing an assay to quantify different methoxypyrazines in green and roasted coffee beans. Show less

  • Technical Expertise

    Sept 2013 - Jul 2020

  • Graduate Research Assistant

    Sept 2012 - Jul 2020
• 

  Intel Corporation

  Sept 2020 - now

  Started working on developing new chemistries and finding solutions for Intel's advanced technology development Gained hands on experience in Semiconductor manufacturing industry coming from a pure organic chemistry background. Worked in wet etch module that included hard mask removal on wafers post Lithography using cutting-edge tools and technology.

  • Module Development Engineer

    Nov 2023 - now

  • TD Module & Integration Yield Engineer

    Sept 2020 - Oct 2023