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Dario Leone

Dario Leone

Research Technician

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location of Dario LeoneBologna, Emilia-Romagna, Italy

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  • Timeline

  • About me

    Flow cytometry and imaging specialist presso Miltenyi Biotec

  • Education

    • Università di Bologna

      2006 - 2008
      Master degree Molecular Biotechnology Cellular and Molecular Biology

    • Università degli Studi di Palermo

      2001 - 2004
      Bachelor in Industrial Biotechnology Cellular and Molecular Biology, industrial food Biotechnology

  • Experience

    • NKI-AVL

      Feb 2009 - Jul 2009
      Research Technician

      Principal techniques and skills:DC isolation from mouse spleen, bone marrow DC in vitro differentiation, FACS staining and analysis, long-term stromal cell culture for production of recombinant protein, protein purification, immunofluorescence staining and analysis, long term imaging with wide field microscopy system (BD pathway 855 system and integrated software including programming macro script for 5 days in-vivo imaging).Animal skill: Bone marrow and spleen isolation, IP and IV injection, blood collection from tail, X ray treatment. Show less

    • MUW Medical University of Wien

      Sept 2010 - Apr 2016
      Phd in Immunology

      Project title1 founded by Marie Curie Action (ESR) TRanSvir network:Circulating LAMP-2 Autoantibody in Focal Necrotizing Glomerulo Nephritis (FNGN) patient Interact with Human Monocytes Derived Dendritic cell (MDDC) modulating the balance between tolerance and immunity influencing their antigen presentation capability.Principal subjects/skills covered:In vitro generation of MDDC from peripheral blood. Pulsing of MDDC with monoclonal or polyclonal anti-Lamp-2 antibody and ideally with autoantibody isolated from patients serum. In-vitro antigen presentation study to identify mechanisms causing breaking of tolerance against MPO and Pr3.Project title2: Characterization of Mononuclear Phagocytic System (MPS) in human kidney. Principal subjects/skills covered:Flow-cytometry and histology are use to classify subpopulation of monocytes, dendritic cell and macrophages that are present in donor or nephrectomy kidney specimen. Show less

    • MUW Medical University of Vienna

      Jul 2016 - Feb 2018
      Post-doc Clinical Institute of Pathology

    • CIML

      Mar 2018 - Mar 2020

      Role of BAD-LAMP in IFN-a production by plasmacytoid Dendritic cell

      • Postdoctoral Research Fellow

        Mar 2018 - Mar 2020

      • Postdoctoral Research Fellow

        Mar 2018 - Mar 2020

    • Miltenyi Biotec

      Mar 2020 - now

      • Flow cytometey and imaging specialist

        Mar 2020 - now

      • Flow cytometry specialist

        Mar 2020 - now

  • Licenses & Certifications

    • Anvanced confocal microscopy course (University of York)

      York University

  • Honors & Awards

    • Awarded to Dario Leone
      Vasculitis foundation grant award Vasculitis foundation Jan 2015 Role of LAMP-2 in Tolerance to ANCA Antigens, $50,000Renate Kain, MD, PhD, Medical University of Vienna, Vienna, Austria
    • Awarded to Dario Leone
      Best oral presentation Medical University of Vienna Jun 2014