Mohamed El Ezzy, Ph.D., CCRP

Experience

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  Lebanese University - Faculty of Sciences

  Sept 2008 - Sept 2010

  Graduate Student

  • I found that Berberis Libanotica, a Lebanese medicinal plant, antiproliferative effects on adult T cell Leukemia.• I climbed to high altitudes to collect the plant. I classified it after detailed dissection and deposited a sample to the herbarium. • Gained a great knowledge in different extraction procedures as well as to prepare the extracts for testing them on leukemic cells.• Acquired skills for culturing cells in suspension and antiproliferative assays. Show less

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  Institut de recherche en immunologie et en cancérologie (IRIC)

  May 2018 - Mar 2019

  First project:• Designed and constructed ERα /ERβ chimeras (Gibson Assembly) which were successfully used shedding new light on the diverse pharmacological properties of antiestrogens.• Developed, validated and executed assays (such as FRET, BRET, western blot, pulldown assays) to monitor Estrogen Receptor and the chimeras’ interactions with SUMO and Ubiquitin in live cells. We also used Luciferase assays and qRT-PCR to monitor the activity of the chimeras in ER negative cell lines.• Discovered a novel mechanism by which of PIAS enhances ER modifications.We did some overexpression and Know downs (shRNA against E3 ligases).• Played a key role in improving the BRET technique into the future era of monitoring multimeric protein assembly in live cell (BRET FECT or Spliced BRET).Second project:• Designed and constructed ER mutants found in breast cancer metastasis resistant to endocrine therapy using site specific mutagenesis.• Uncovered a novel mechanism by which ER mutants activate transcription in the presence full antagonists and inverse agonists.• Designed a High Throughput chemical screen to measure the efficacy and potency of newly developed antiestrogen and antiandrogen HDACis hybrids.• Significant Contributions to quality data for Grant applications.• Trained Graduate students and postdoctoral fellows in mastering techniques needed fortheir projects. Show less

  • Doctoral Student

    Sept 2012 - Mar 2019

  • Academic Tutor

    May 2018 - Jul 2018
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  TTEC

  Apr 2019 - Aug 2022

  Customer Service Support Specialist

  Customer Experience, Customer Satisfaction, Team management, Communication, CRM, Ticket Sales, Social Media, Problem Resolving, Soft Skills, Excel sheet coding and pivot tables.

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  INTERNATIONAL ACADEMY OF CLINICAL RESEARCH LTD

  Jul 2020 - May 2022

  Clinical Trail Assistant

  • Worked in Phase IIb/III metabolic syndrome and LDL-C and Phase IV diabetes studies• Wrote Protocols synopsis, Investigator Brochure and prepared SOPs and REB submissions for clinical trials.• Designed, wrote and verified Case Report Form (Study Preparation and laboratory data entry in CRF), Informed Consent Form (ICF), SDV as part monitoring internship.• Filled and verified the IND application, NDA, sNDAs and, QIU.• Remote Internship Experience in Source Documents Review and Source Documents Verification. Show less

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  Kisoji Biotechnology Inc

  Mar 2022 - Sept 2022

  Research Scientist in Assay Developement

  • Developed new assays such as the FACS based macrophage phagocytosis assays for testing antibody activity which helped the company’s corporate presentation.• Adapted in house reporter assays for antibody screening such as ADCC and ADCP assays (Antibody dependent Cell Cytotoxicity and Phagocytosis assays).• Demonstrated an excellent mastery using multicolor flow cytometry, ELISA, cell binding assays, IP-western blot and qRT-PCR.• Showed a robust experience in cell culturing (Thawing, Passaging and Cell Freezing) of various tumor cell lines (adherent and cell in suspension).• Managed a project for developing antibodies against GPCR receptors.• Prepared reports and high-quality data for monthly data reviews.• Maintained Good Laboratory Practices (GLPs) and in creating and maintaining Standard Operating Procedures (SOPs). Show less

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  Paraza Pharma Inc.

  Nov 2022 - now

  Research Scientist