Experience

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  Max planck institute of molecular physiology

  Oct 2014 - Feb 2015

  Bachelor thesis student

  The goal of my bachelor thesis was to set up and optimize the purification of a novel protein Ivy1 from E. coli. and to identify the effect of this protein on the small GTPase Ypt7 by using SDS-PAGE, HPLC, and ITC.Our work was published as: Rai, A., Oprisko, A., Campos, J., Fu, Y., Friese, T., Itzyen, A., Goody, R., (2016). "bMERB domains are bivalent Rab8 family effectors evolved by gene duplication." Elife 5.During this project, I successfully performed: • Cultivation of E. coli and protein expression in the E. coli cells• DNA cloning by using PCR • Purification and analysis of proteins by using size exclusion chromatography (SEC), affinity chromatography, high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC) and mass spectrometry Show less

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  Max-planck institute for molecular physiology, dortmund, germany

  Apr 2015 - May 2015

  Graduate student research assistant

  In this internship, I continued to research the project in my bachelor thesis and successfully purified a novel protein.

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  Dkfz-zmbh allianz

  Jan 2016 - Feb 2016

  Student research assistant

  In this project, I successfully performed a number of biochemical and molecular biological techniques:• Growth of Escherichia coli analysis by means of omega spectrometer, DNA cloning by using PCR • Isolation of plasmid from E. coli via minipreparation• Plasmid analysis using agarose gel electrophoresis and UV / Vis spectrometer • Expression test of proteins in E. coli cells • Protein analysis by using western blot and immunoprecipitation

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  Dkfz german cancer research center

  Apr 2016 - Jun 2016

  Student research assistant

  In this project, I successfully performed a number of molecular biology and biochemistry techniques:• Bacterial transformation and plasmid purification• Site-directed mutagenesis• Protein production and purification using an ÄKTA purification system - Immobilizedmetalaffinitychromatography(His-column) - GST-taggedproteinpurification - Sizeexclusionchromatography• Reducing/non-reducing SDS-PAGE• Western blotting• Blue native PAGE

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  Basf

  Sept 2016 - Nov 2016

  Management-trainee

  Department of Technology, Performance Chemical; Unit of Product Safety and Quality ManagementI successfully completed my following tasks:• Literatur research on the type and content of degradation products in plastics as well as their migration potential related to gamma irradiation and thermal deformation• Collection of toxicological data as related to degradation products in plastics• Evaluation of degradation products in terms of environmental and safety aspects. • Evaluation in accordance with current regulations on food contact materials that apply to EU, USA, and China.• Responding to customer questions on different topics related to product safety • Summarizing the work during the internship and presenting results internally Show less

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  University of california, berkeley

  Jan 2017 - May 2017

  Student research assistant

  Preparation of the project in my master thesis. During this time, I successfully performed:• Cultivation and infection of Insect cells and mammalian cells • Proteins expression and purification• Setup and optimization of guanine nucleotide hydrolysis/exchange assays and their analysis by using HPLC

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  University of california, berkeley

  Sept 2017 - Mar 2018

  Master thesis student

  The mTOR complex 1 (mTORC1) is a master growth regulator in mammalian cells. In my master thesis, I proposed a novel model for the amino acid-sensitive Rag GTPases cycling upstream of mTORC1 activation via verifying the regulation of the factors alone or in combination.Our work was published as: Su, M. Y., Morris, K. L., Kim, D. J., Fu, Y., Lawrence, R., Stjepanovic, G., Zoncu, R., Hurley, J. H. (2017). "Hybrid Structure of the RagA/C-Ragulator mTORC1 Activation Complex." Mol Cell 68(5): 835-846 e833.More publications are in preparing.During the time, I successfully performed a number of biochemical and molecular biological techniques:• Cultivation and infection of Insect cells and mammalian cells • MBP-, GST- or His-tagged proteins expression and purification from the different cell cultures• Plasmids design and new DNA constructs creation by using PCR• In-vitro and in-vivo analysis of guanine nucleotide hydrolysis/exchange on proteins by using the optimized methods• Transfection of mammalian cells by using RNA-Interference • In vivo- and in vitro analysis the interaction and location of the proteins by using immunofluorescence, immunoblotting, SDS-PAGE and FPLC Show less

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  Johann wolfgang goethe-universität frankfurt am main

  Oct 2018 - now

  Researcher phd student