Himsheela Karki

Himsheela karki

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location of Himsheela KarkiFrederick, Maryland, United States
Followers of Himsheela Karki220 followers
  • Timeline

  • About me

    Analytical Chemist

  • Education

    • Morgan state university

      2020 - 2023
      Bachelor of science - bs chemistry

      Outstanding Poster Presentation in Biology/ Chemistry 27th Annual Undergraduate and Graduate Research Symposium at Morgan State University April 2022.Awarded the Maryland Section of the American Chemical Society Award 2022.Honors Student.Phi Theta Kappa Member.The National Society of Leadership and Success (NSLS) member.

    • Baltimore city community college

      2016 - 2018
      Associate of arts and sciences - aas biotechnology

      Awarded the President's Award for Best Overall Intercollege Science Project at the 2018 STEM Symposium.Awarded the Exceptional Internship Award 2019 from Baltimore City Community College.Awarded the ACS Award 2019.Dean’s list for academic achievement (2016-1019).Vice President of BCCC Biotech Student Society.Member of International Student Club, Phi Theta Kappa Honor Society, Science Club, and Environment Club.

  • Experience

    • University of maryland school of medicine

      Jun 2018 - Dec 2018
      Research assistant internship

      • Participated in a collaborative research project with a postdoctoral fellow, investigating the impact of varying pressure conditions on traumatic brain injuries relevant to aeromedical evacuation.• Performed sample preparation, western blots, tissue handling and slicing, immune histochemistry, gel electrophoresis, and PCR.• Collaborated with team members to achieve research objectives, and actively participated in group discussions to enhance project outcomes.

    • Graybug vision, inc.

      Sept 2019 - Jan 2020
      Laboratory technician

      • Oversaw the preparation of a production suite by setting up the room, calibrating equipment, performing extensive cleanings, and formulating buffers at both small scales to a functional batch size for pre-clinical and clinical trials.• Performed routine analytical tests such as vacuum filtration, titration, pH, and conductivity measurements on formulated buffers.• Coordinated with senior lab members to maintain optimal inventory, reduced overstocking, and eliminated stock shortages by performing inventory for each item, using the 5S principle, cycle count and strictly adhering to real-time consumption. Show less

    • Morgan state university

      Aug 2021 - May 2023
      Research assistant (chemistry)

      • Conducted research project focused on designing and synthesizing- Fluorescent Small Organic molecules- aimed at sensing and recognizing a range of transition and toxic metal ions.• Synthesized 4+ Rodhamine-6G based compound, among which two showed selectivity for lead and copper ion.• Proficiently operated laboratory instruments, including the CEM microwave reactor, UV/Vis spectrometer, and fluorescence spectrometer.

    • Astrazeneca

      Jul 2023 - Dec 2023
      Production technician

      • Executed biopharmaceutical manufacturing operations using equipment such as, chromatography skids, CIPs, SIPs, and in-process testing instruments (SOLO VPE, pH meter, osmometer, pumps, etc.) by following SOPs. • Involved in Downstream operations of 10+ batches of monoclonal antibodies, at 2000L and 500L batch scale, within AstraZeneca's clinical large-molecule drug portfolio. • Proficient in performing tasks such as steam sterilization, clean in place, manual cleaning, and equipment sanitization.• Maintained records of manufacturing activities in adherence to approved current good manufacturing practices (cGMP) documents and regulatory requirements. • Proactively ensured the readiness of equipment and materials for processing, contributing to the smooth workflow. • Troubleshoot system-related production complications in a fast-paced work environment. • Upheld a commitment to a safe and environmentally sustainable work environment, aligning with AstraZeneca standards.• Demonstrated proficiency in utilizing MS Office tools and AstraZeneca enterprise systems for efficient workflow management. Show less

    • Baltimore city department of public works

      Jan 2024 - Aug 2024
      Chemist ii

      • Conducted routine wastewater analyses, including TSS, VSS, COD, BOD, TOC, Turbidity, and Alkalinity, achieving precise water quality monitoring throughout the treatment stages.• Operated the SEAL AQ2+ discrete analyzer daily to detect and quantify Ammonia, Nitrate, Nitrite, Orthophosphate, Total Phosphate, and Total Kjeldahl Nitrogen in wastewater samples.• Documented test results and quality control data in the LIMS, generating detailed and accurate water quality reports.• Provided training and mentorship to chemists, microbiologists, and lab assistants, enhancing their operational skills and ensuring consistent, accurate results.• Secured three permits for the lab while coordinating effectively with the team. Enhanced accuracy by recording all parameters, leading to the elimination of potential errors and prevention of quality issues. Show less

  • Licenses & Certifications

    • Lab animal science

      Baltimore city community college
      Jan 2018