Johny Ijaq, Ph.D.

Experience

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  Osmania University

  Jan 2013 - Dec 2019

  Doctoral Student

  𝗧𝗶𝘁𝗹𝗲: Machine Learning Based Evaluation of Human Hypothetical Proteins and Validation of Their Expression.𝗧𝗵𝗲𝘀𝗶𝘀: My PhD work mainly focused on studying human hypothetical proteins (HPs), a group of proteins for which there is no experimental evidence of translation and functional characterization. Different bioinformatics tools and methods were used for the functional annotation of hypothetical/uncharacterized protein sequences. Different available functional annotation methods were evaluated using machine learning based models. Later, to check whether or not these sequences are translatable, proteomics based approach was used to provide the analytical basis of their identification. My study established most of these hypothetical sequences as non-coding RNAs or the products of pseudogenes rather than proteins. However, confirming the expression of few HPs at protein level made it clear that these 'hypothetical' or 'uncharacterized' proteins cannot be completely ignored.PMID: 25873935PMID: 30621574https://doi.org/10.1007/s42485-020-00030-1 Show less

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  University College for Women, Osmania University

  Sept 2015 - Sept 2017

  Teaching Assistant

  Taught Biochemistry, Cell biology, and Molecular biology courses to MSc students.

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  Indian National Science Academy - INSA

  May 2018 - Jul 2018

  Summer Research Fellow

  Project: Identification of differentially expressed genes in Staphylococcus aureus in response to antibiotic exposure.

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  Centre for Cellular and Molecular Biology

  Jan 2019 - Jun 2019

  Guest Researcher

  Project: Identification and characterization of human hypothetical proteins from cancer cell lines using proteomics and bioinformatics approaches.• Sample processing for proteomics• SDS-PAGE• Protein profiling using LC-MS/MS method • Data acquisition using Thermo Q Exactive HF Orbitrap System• Proteome discoverer• Label free quantification

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  Centre for Cellular and Molecular Biology

  Sept 2020 - Dec 2020

  Research Trainee
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  University of Hyderabad

  Apr 2021 - Apr 2023

  Research Associate

  • Metabolite profiling from different types of biological matrices (biofluids, cell lines, bacterial cultures) using GC-MS and LC-MS/MS-QTOF • Contributing to set-up of standardized protocols for sample preparation• Operating and troubleshooting chromatographic/mass spectrometry equipment• Carrying out sample analyses using the targeted and/or untargeted approach• Contributing to set-up of standardized LC-MS methods for targeted analysis• Performing data analysis using the existing bioinformatics platform (MetaboAnalyst)• Keeping an up to date written and electronic record of laboratory work• Contributing to maintenance of adequate stocks in the laboratory (consumables such as chemicals, gases, disposables)• Helping ensure the functionality of key platform apparatus (service/maintenance) by liaising with the platform staff in a timely manner Show less

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  Yenepoya University

  Oct 2023 - now

  Scientific Officer