Sergio Aoki

Experience

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  UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho"

  Jan 1997 - Jan 2001

  Veterinary Medicine degree

  - Scientific Initiation FAPESP fellowship (1998-1999)- Polymorphisms analysis from Exons 7/8 and Intron VII from Bovine Follicle Release Receptor Gene by SSCP (Single Strand Conformation Polymorphism) technique. Experience in nucleic acids purification, PCR, Horizontal and vertical electrophoresis, SSCP and Sanger Sequencing.- Scientific Initiation FAPESP fellowship (2000-2001)- Pathogens identification in bovine semen by multiplex fluorescent PCR (Polymerase Chain Reaction) technique. Experience in Leptospira spp. and Campylobacter bacterial culture. Experience in nucleic acids purification from semen samples. Fluorescent Multiplex PCR detection of Leptospira spp., Campylobacter jejuni, Brucella abortus, Haemophillus somnus, BHV-1 and BVD virus. Capillary Electrophoresis. Show less

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  UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho"

  Jan 2002 - Jan 2006

  Doctor degree in Biological Sciences- Genetics

  - Direct doctorate fellowship (2002-2006)- Genetic and Epidemiological characterization of isolated Bovine Herpesvirus Type 1 (BHV-1). Harvesting and packaging of blood and trigeminal ganglia during the routine of bovine slaughterhouse for BHV-1 virus isolation. Anti-BHV-1 antibodies detection by ELISA. E. coli PCR fragments cloning and Sanger Sequencing. Use of bioinformatics tools to fragments, phylogeny and epidemiology analysis. - Cooperation in other research projects from LBBMA lab group: Target Nucleotide Exchange-TNE, Serial Analysis of Gene Expression-SAGE, Microarrays, Real Time PCR to validate Gene Expression data, Microsatellites, PCR Visceral Leishmaniosis diagnosis, PCR-Restriction Fragment Length Polymorphism, Sequence Characterized Amplified Regions-SCARs Taenia saginata e Taenia solium. Show less

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  DNAPTA Biotecnologia

  May 2008 - Nov 2016

  Scientific Director

  - Company guidance for scientific and commercial activities.- Strategic planning and implementation of academic and commercial partnerships.- Management of Laboratorial technical staff.- Technical reports, patents, and scientific articles.- Obtaining funding to support activities of R, D & I (FINEP/FAPESP/CNPq/BNDES).- Technical-economic feasibility evaluation and presentations to customers.- Development and coordination of innovative projects in developing Aptamers, Antibodies, Antigens, Recombinant Proteins, Serologic Assays, Molecular Diagnosis and Biosensors facing the treatment, prevention and diagnosis of human and animal diseases.- Standardization and implementation of purification protocols of DNA/RNA, PCR, qPCR, emPCR, NGS sequencing, Dot Blot, Western Blot, ELISA, Lateral Flow Immunochromatography, Cell Culture, Purificaton/Modification of proteins/peptides/antibodies and Biosensors. Show less

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  Zoetis

  Dec 2016 - now

  • Principal Scientist

    Oct 2024 - now

  • Senior Research Scientist

    Dec 2016 - Oct 2024