Brian Estvander, MS

Experience

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  AbbVie

  Jan 2002 - Jan 2007

  Associate Pharmacologist

  • Supported HTS efforts for all in vitro radioligand functional, saturation, and competition binding assays utilizing I-125, 3H, and 35S and the Calcium-Influx FLIPR assay in Pain Therapeutics/Cognitive Disorders/Obesity. • Developed novel radioligand binding assay unveiling drug potency equivalence for solubilized and intact membrane receptor and awarded Global Pharmaceutical Research & Development Innovation and Teamwork awards.• Advanced Radiation Safety certified. Controlled Drug Agent sponsored by DEA. • Awarded the first Academic-Abott Labs Scholarship at Rosalind Franklin University of Medicine and Science in the Department of Pharmacology. Partially completed Ph.D. coursework and lab rotations. Show less

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  Nanotope, Inc.

  Jan 2008 - Jan 2012

  Manager of Tissue Regeneration, Biological Studies

  Built biology and chemistry lab from scratch, designing lab, plumbing, electrical, HVAC, and procurement of capital equipment and consumables for in vitro designed assays for regenerative tissue engineering.• Developed quantifiable and reproducible novel 3D immunocytochemistry assay to predict efficacy of peptide amphiphile for studies in animal models in Spinal Cord Injury. • Designed quantifiable and reproducible 3D in vitro scratch assay to predict efficacy of peptide amphiphile in wound healing and cartilage regeneration and piloted in vivo studies with collaborative companies.• Appointed Visiting Scholar at Northwestern University and obtained certification for spinal cord surgery in rodent models at Rutgers University Spinal Cord Regenerative Institute. Show less

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  Fresenius Kabi

  Jan 2013 - Jan 2014

  Product Development Project Manager

  Hired, trained and led five team members and managed GMP analytical and biochemistry cell culture labs in support of peptide injectables. Co-authored method development and validation/transfer protocols. Collaborated with CRO for IQ/PQ/OQ of internal methods/equipment validation. Pharmacological, cell biology, and analytical chemistry SME for company acquisition and CRO opportunities.

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  Baxter International Inc.

  Jan 2014 - Jan 2015

  Project Manager II, Surgical, Anesthesia and Critical Care

  Contracted to assume role during employee leave of absence. Led PLC meetings with cross-functional teams across Latin America, Middle East, and Asian Pacific regions. • Streamlined product life cycle for transition of anesthetic glass to aluminum container and governed successful global transition across LATAM, Asia, and New Zealand.

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  Mylan

  Jan 2015 - Jan 2016

  Site Quality Control Specialist

  Ensured optimum pharmaceutical efficacy through rigorous quality testing systems oversight for stability and dissolution statistical analysis and data trending, noting OOS/OOT anomalies. Developed and implemented QA protocols, risk management processes, and SOPs for internal and external oversight operations and audits. • Authored and implemented Cleaning Validation Master Plan for removable packaging parts.• Managed and executed all environmental QC site procedures including facility temperature, humidity, microbial surface and air testing, cleaning validations, water testing, and pest control. • Commended for excellent work in upholding consistent compliance with ICH guidelines. Show less

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  BNG-Enterprises Inc

  Jan 2016 - Jan 2019

  Vice President Operations, Inspector

  Managed all aspects of branding and marketing, operations, and quality assurance and control. Specialized in providing structural and component inspection services for residential and light commercial properties including interior and exterior structure, HVAC, insulation, plumbing, electrical, roofing, chimney, and decking.• Achieved 100% customer satisfaction with detailed visual and written documentation and final in-person walk-through.• Earned industry association designations including ICA and InterNACHI, NRSB radon testing, and IAC2 mold testing and remediation certifications. Show less

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  Thermo Fisher Scientific

  Jan 2019 - Jan 2023

  Ensured product quality through rigorous analytical testing on raw materials, intermediates, and finished products to ensure compliance with established specifications and regulatory requirements. Collaborated with cross-functional teams across manufacturing, R&D, and QA to support continuous improvement initiatives.• Authored certificate of analysis for product release.• Forecasted time and resources costs for testing method using data analytic software R and process mapping using Alteryx.• Reduced testing time and cost from two days to four hours with existing resources by developing and validating small molecule/protein conjugation method.• Developed and validated novel well plate binding method; reduced testing time from four hours to one hour and reduced user learning curve, improving intermediate test precision. • Improved consumables purchase and storage Kanban by creating true database using SQLite instead of Excel filing system for material and reagent tracking. • Partnered to establish GHS labeling standard of reagents for all Rockford TFS site. Show less SME for two membrane protein research projects. Tracked and allocated consumables, reagents, and capital equipment budget. • Point person for Styrene Maleic Acid Lipid Particles (SMALP) research project plans and established working NDA with university to leverage technology. Identified SMALP variants for solubilization and purification of several membrane protein receptors classes.• Led development and rollout of method validation and specifications for executing in-depth study of gold standard membrane protein and reagent kit for customer use in cryo-EM and nMS.• Established and validated more efficient immunoprecipitation method for the human 80S ribosome for cryo-EM application in conjunction with MCR grant research. Show less

  • Quality Control Chemist II

    Jan 2019 - Jan 2023

  • R&D Protein Scientist II

    Jan 2019 - Jan 2022

  • Quality Control Chemist I

    Jan 2019 - Jan 2020
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  Outlier

  Jan 2024 - now

  AI Systems and LLM Quality Engineer

  Contract to develop AI prompting for peer-reviewed biology literature for method and results annotations. Bridge gap between worlds of biology, statistical analysis and AI using NLP, Bee MM, and LaTex code.• Provided open source contributions to GitHub and Kaggle using coding languages and data base structure design software.