Collin Stabler, Ph.D., P.E., RAC

Michael Brown Penn-GSK Postdoctoral Fellowship University of Pennsylvania / GSK Aug 2018

Lung Repair and Regeneration Consoritum (LRRC) Travel Award Lung Repair and Regeneration Consortium (LRRC) Jun 2016

The Hartwell Foundation Fellowship The Hartwell Foundation May 2016 http://thehartwellfoundation.org/2015/2015_Fellows.shtml

Temple University College of Engineering Poster Competition, 1st Prize Temple University Mar 2014 ABSTRACT:Chronic lung diseases are the second leading cause of death in the modern world. For these patients the only curative option would be single/double lung transplantation. The demand for lung transplantation is gradually surpassing the available supply of donor lungs, which highlights the urgent need for tissue-engineering based lung repair and/or replacement strategies. Decellularization has shown promise as an enabling technology platform for development of novel regenerative… Show more ABSTRACT:Chronic lung diseases are the second leading cause of death in the modern world. For these patients the only curative option would be single/double lung transplantation. The demand for lung transplantation is gradually surpassing the available supply of donor lungs, which highlights the urgent need for tissue-engineering based lung repair and/or replacement strategies. Decellularization has shown promise as an enabling technology platform for development of novel regenerative approaches with a potential of generating organs for transplantation. Due to the complexity of the lungs only recently have attempts been made in order to decellularize the lungs as a whole organ. Maintaining the structure of the heart and its various blood vessels during and after dissection is important in order to allow for physiologic flow through the respiratory system. Decellularization was carried out with perfusion of 0.1% TritonX-100 for 18hrs and 2% Sodium Deoxycholate for 18hrs. Immunohistochemistry (Hematoxylin and Eosin (H&E), Masson’s Trichrome, Verhoeff’s Van Gieson) and DNA quantitization were used to analyze quality of decellularization. Mouse embryonic stem cells (E14) were seeded through the airways, and rat lung microvessel endothelial cells seeded through the vasculature. Here we have shown that optimization of seeding parameters (e.g. – supplementation of basement membrane proteins) can be quantified for development of an efficient re-seeding strategy. We believe that our analysis techniques will aid in the progress towards a fully functional bioengineered lung. Show less

NASA GSRP Fellowship National Aeronautics and Space Administration (NASA) Aug 2010