Aswini Krishnan (Dr. Rer. Nat)

Experience

• 

  Kroto Research Institute · North Campus

  Feb 2009 - Aug 2009

  MSc Research Project

  Project title: Investigation of the ability of collagen IV in the enhancement of neuronal cell growthMy project focussed on the effect of different biomaterial surfaces on the adsorption and subsequent functionality with respect to cell attachment and proliferation of the extra cellular matrix protein collagen IV. Quantification studies for the adsorption of collagen IV on biomaterial surfaces glass, PLLA and acrylic acid using ELISA showed that there was no significant difference in the adsorption of collagen IV among the surfaces investigated. F-actin staining studies using confocal microscopy in NG-108 cells indicated that there was no appreciable difference in the attachment of cells on the different surfaces. Also, the dependence of cell attachment on beta1 integrin, a high affinity receptor for collagen IV was investigated by integrin blocking studies. This study implicated the role of beta-1 integrin on cell attachment. The cell number analysis studies suggested that collagen IV coating had no significant effect on cell proliferation. Show less

• 

  The University of Sheffield

  May 2010 - Oct 2010

  Voluntary Research Technician

  - ELISA based quantification of interleukins (IL-8, IL-6 and IL-1α) released by keratinocytes and fibroblast cells maintained in different types of conditioned media.

• 

  Cancer Research UK Beatson Institute

  Nov 2010 - Feb 2012

  Scientific Officer

  1. Scientific officer, Urology Research Laboratory, Supervisor Prof. Hing Leung. (June 2011 – February 2012)- Investigation of centrosome kinase SIK2 as a novel therapeutic target for prostate cancer- Study the phenotypic cellular effects of SIK2 knockdown using siRNA in various prostate cancer cell lines2. Scientific officer, Ubiquitin Signalling Laboratory, Supervisor Dr. Danny Huang. (November 2010 – May 2011)- Expression (in E.coli), purification and assembly of protein for crystallisation studies Show less

• 

  Institute for Stem Cell Science and Regenerative Medicine (DBT-inStem)

  Nov 2012 - Apr 2014

  Junior Research Fellow

  - Establishment and maintenance of the Cell Culture Laboratory- Establishment of primary neuron culture from rat embryo brains- Identification of mRNA targets of FMRP amygdala and hippocampus of one month old rats

• 

  Johannes Gutenberg University Mainz

  Aug 2014 - Sept 2019

  Researcher PHD Student

  - Genomic and proteomic analysis of normal vs tumor vs metastatic patient tissues with Papillary Thyroid Cancer (PTC)- Validation of identified genomic and proteomic candidates as oncogenic alteration and/or therapeutic targets through extensive cell biological and biochemical assays- The study identified and validated a novel oncogenic fusion, as well as proteins involved in the Ubiquitination pathway as potential therapeutic markers in PTC- Cell banking of patient derived cells- Documentation of lab activity and data- Provide training and supervision to team members Show less

• 

  University of Zurich

  Jun 2020 - Dec 2022

  Postdoctoral Researcher

  - Cross-functional collaboration with University Hospital Zurich (USZ) and drug screening platform (Nexus, ETH), to plan project strategies and execute project goals- Project co-ordination and management involving patient sample screening and acquisition - Data management and statistical analysis of clinical data for the evaluation of prognostic outcomes- Optimize pre-drug screening processes, estimate and ensure material availability for planned experiment- Contribute to reviewing, editing and submission of scientific reports- Develop and present scientific data updates/progress to diverse audience Show less