Nirakar Basnet

Experience

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  Max Planck Institute of Biochemistry

  Jun 2014 - Jan 2020

  PHD

  Conducted in-depth investigations into the role of the coiled-coil protein SSNA1, enhancing understanding of its critical influence on microtubule remodeling and axon branch formation.Utilized advanced imaging methodologies such as Single Particle Electron Microscopy (EM) and Single Particle Tomography to examine cellular structures at high resolutions, facilitating groundbreaking discoveries in cellular architecture.Performed Cryo-EM structure determination of microtubules in conjunction with microtubule-binding proteins, significantly advancing knowledge of their functional interactions and dynamics. Show less

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  National Heart, Lung, and Blood Institute

  Jun 2020 - Apr 2022

  Postdoctoral Researcher

  Specialize in advanced imaging techniques including Cryo-Electron Microscopy (Cryo-EM) and Cryo-Electron Tomography for structural analysis, with a strong focus on understanding molecular mechanisms underlying axon branching.Proficient in employing Single Particle Analysis and Correlative Cryo-Electron Tomography, providing detailed insights into complex biological structures and processes.Skilled in sample preparation using Focused Ion Beam milling (FIB-SEM) and Cryo-Liftout techniques, optimizing the visualization of molecular interactions at high resolutions.Extensive experience in data analysis and reconstruction of Single Particle data and sub-tomograms, using such as Relion, EMAN2, IMOD, cryoSPARC, Frealign, and Dynamo, ensuring precise and accurate structural interpretation.Demonstrated expertise in protein expression and purification from both mammalian and bacterial systems, delivering high-quality samples crucial for successful structural studies. Show less

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  Institute for Protein Innovation

  Apr 2022 - now

  Postdoctoral Researcher

  Expertise in expressing and purifying G protein-coupled receptors (GPCRs) using mammalian systems to ensure high-quality protein samples for research and development.Lead the development of conformation-specific antibodies targeting GPCRs by leveraging advanced yeast display techniques. This includes designing and executing screening strategies to enhance antibody specificity and efficacy.Conduct comprehensive antibody binder repertoire analyses utilizing next-generation sequencing (NGS), enabling precise selection and optimization of antibody candidates.Employ a variety of assays to validate antibody specificity, including cell display techniques, surface plasmon resonance (SPR), and other binding assays, to ensure robust performance in diverse applications.Evaluate the functional impact of antibodies on GPCR signaling pathways, assessing their potential for therapeutic applications and contributing to our understanding of receptor biology. Show less